Hirose K, Kobayashi H, Ito N, Kawasaki Y, Zako M, Kotani K, Ogawa H, Sato H
Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
J Vet Med B Infect Dis Vet Public Health. 2003 Sep;50(7):347-51. doi: 10.1046/j.1439-0450.2003.00681.x.
Nasal swabs of 293 calves were examined for Mycoplasma. The samples were collected from calves affected with respiratory diseases on 71 farms in various parts of Japan between 1996 and 1997. Mycoplasma bovirhinis was isolated from 47 of 293 calves (16.0%). Mycoplasma alkalescens, M. bovis, M. arginini, M. bovigenitalium and Acholeplasma spp. were isolated from 19 (6.5%), seven (2.4%), four (1.4%), four (1.4%) and 18 (6.1%) calves, respectively. Pasteurella multocida and P. haemolytica were isolated from 60% of Mycoplasma-positive calves. However, other bacteria were not isolated from calves. To evaluate the antimicrobial susceptibility of their isolates, 68 M. bovirhinis, 21 M. alkalescens and 10 M. bovis strains were examined for 12 antimicrobial agents. All isolates showed higher susceptibility to tiamulin than to the other drugs used in the study. However, erythromycin had no effect on any of the Mycoplasma strains studied. The field isolates were less susceptible than the type strains to some drugs, such as spiramycin, oxytetracycline and tylosin.
对293头犊牛的鼻拭子进行了支原体检测。这些样本于1996年至1997年期间从日本各地71个农场患有呼吸道疾病的犊牛中采集。从293头犊牛中的47头（16.0%）分离出牛鼻支原体。分别从19头（6.5%）、7头（2.4%）、4头（1.4%）、4头（1.4%）和18头（6.1%）犊牛中分离出碱性支原体、牛支原体、精氨酸支原体、牛生殖道支原体和无胆甾原体属。多杀性巴氏杆菌和溶血巴氏杆菌从60%的支原体阳性犊牛中分离出来。然而，未从犊牛中分离出其他细菌。为了评估其分离株的抗菌药敏性，对68株牛鼻支原体、21株碱性支原体和10株牛支原体菌株进行了12种抗菌药物检测。所有分离株对泰妙菌素的敏感性均高于本研究中使用的其他药物。然而，红霉素对所研究的任何支原体菌株均无作用。田间分离株对某些药物（如螺旋霉素、土霉素和泰乐菌素）的敏感性低于标准菌株。
