Tschochner H, Sayre M H, Flanagan P M, Feaver W J, Kornberg R D
Department of Cell Biology, Fairchild Center, Stanford University School of Medicine, CA 94305.
Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11292-6. doi: 10.1073/pnas.89.23.11292.
Yeast RNA polymerase II initiation factor e was purified to homogeneity and identified by biochemical criteria as the counterpart of human transcription factor IIB. Factor e was essential for initiation of transcription from yeast and mammalian promoters in a reconstituted yeast transcription system. Activity resided in a single polypeptide of approximately 41 kDa, identified by peptide sequence analysis as the product of the SUA7 gene. Factor e interacted specifically with RNA polymerase II, consistent with a proposed role in determining the start site of transcription.
酵母RNA聚合酶II起始因子e被纯化至同质，并通过生化标准鉴定为人类转录因子IIB的对应物。在重组酵母转录系统中，因子e对于从酵母和哺乳动物启动子起始转录至关重要。活性存在于一条约41 kDa的单一多肽中，通过肽序列分析鉴定为SUA7基因的产物。因子e与RNA聚合酶II特异性相互作用，这与它在确定转录起始位点方面的假定作用一致。
