McGrath Deborah, Costanzo Stephen P, Manchester Robert J, Kaiser James J, Norton Susan E, McCormick Patrick J
Bausch & Lomb Incorporated, Rochester, NY, USA.
Eye Contact Lens. 2003 Oct;29(4):245-9. doi: 10.1097/01.icl.0000090883.42719.C4.
This study reports the effect of organic soil on the antimicrobial activity of four commercially available multipurpose contact lens care solutions used in no-rub regimens as determined by a modified International Organization for Standardization 14729 (ophthalmic optics-contact lens care products-microbiological requirements for products and regimens for hygienic management of contact lenses) Stand Alone Test procedure.
Testing was performed with organic soil consisting of a mixture of heat-killed yeast cells and heat-inactivated bovine serum or these components added separately. The organic soil was mixed with the challenge microorganisms before addition to the solution, added to the solution after addition of the challenge microorganisms, or added to the solution before addition of the challenge microorganisms. A final concentration of 0.4% v/v organic soil and 1 x 105 to 1 x 106 cfu/mL challenge microorganisms was realized in all test cases.
The antimicrobial activity of no-rub multipurpose lens care solutions was reduced in the presence of added organic soil. The extent of reduction varied with the type of organic soil, the method of addition of the organic soil to the solution, the challenge organism, and the solution tested.
The type of organic soil used and the method of addition of organic soil to the lens care solution may affect antimicrobial activity as determined by the primary criteria of the International Organization for Standardization Stand Alone Test procedure. The overall reduction in antimicrobial activity depends on the solution and organism.
本研究报告了通过改良的国际标准化组织14729（眼科光学——隐形眼镜护理产品——隐形眼镜卫生管理产品和方案的微生物要求）独立测试程序，有机土壤对四种市售无揉搓方案使用的多功能隐形眼镜护理溶液抗菌活性的影响。
使用由热灭活酵母细胞和热灭活牛血清混合物组成的有机土壤进行测试，或者分别添加这些成分。有机土壤在添加到溶液之前与挑战微生物混合，在添加挑战微生物之后添加到溶液中，或者在添加挑战微生物之前添加到溶液中。在所有测试案例中，有机土壤的最终浓度为0.4%（v/v），挑战微生物的浓度为1×10⁵至1×10⁶ cfu/mL。
在添加有机土壤的情况下，无揉搓多功能隐形眼镜护理溶液的抗菌活性降低。降低程度因有机土壤类型、有机土壤添加到溶液中的方法、挑战微生物和测试溶液而异。
所使用的有机土壤类型以及有机土壤添加到隐形眼镜护理溶液中的方法，可能会影响根据国际标准化组织独立测试程序的主要标准所确定的抗菌活性。抗菌活性的总体降低取决于溶液和微生物。
