来自集胞藻PCC 6803且缺乏PsaF和PsaJ亚基的光系统I三聚体结合一个由17个单位组成的IsiA环。
Photosystem I trimers from Synechocystis PCC 6803 lacking the PsaF and PsaJ subunits bind an IsiA ring of 17 units.
作者信息
Kouril Roman, Yeremenko Nataliya, D'Haene Sandrine, Yakushevska Alevtyna E, Keegstra Wilko, Matthijs Hans C P, Dekker Jan P, Boekema Egbert J
机构信息
Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
出版信息
Biochim Biophys Acta. 2003 Oct 17;1607(1):1-4. doi: 10.1016/j.bbabio.2003.08.002.
We report a structural characterization by electron microscopy and image analysis of a supramolecular complex consisting of Photosystem I (PSI) and the chlorophyll-binding protein IsiA from a mutant of the cyanobacterium Synechocystis PCC 6803 lacking the PsaF and PsaJ subunits. The circular complex consists of a central PSI trimer surrounded by a ring of 17 IsiA units, one less than in the wild-type supercomplex. We conclude that PsaF and PsaJ are not obligatory for the binding of the IsiA ring, and that the size of the PSI complex determines the number of IsiA units in the ring. The resulting number of 17 copies implies that each PSI monomer has a different association to the IsiA ring.
我们通过电子显微镜和图像分析报告了一种超分子复合物的结构特征，该复合物由来自集胞藻PCC 6803突变体的光系统I（PSI）和叶绿素结合蛋白IsiA组成，该突变体缺乏PsaF和PsaJ亚基。环状复合物由一个中心PSI三聚体组成，周围环绕着一个由17个IsiA单元组成的环，比野生型超复合物少一个。我们得出结论，PsaF和PsaJ对于IsiA环的结合不是必需的，并且PSI复合物的大小决定了环中IsiA单元的数量。所得的17个拷贝数意味着每个PSI单体与IsiA环有不同的结合方式。
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