Evdokimova Svetlana A, Nokhaeva Vera S, Karetkin Boris A, Guseva Elena V, Khabibulina Natalia V, Kornienko Maria A, Grosheva Veronika D, Menshutina Natalia V, Shakir Irina V, Panfilov Victor I
Department of Biotechnology, Faculty of Biotechnology and Industrial Ecology, D. Mendeleev University of Chemical Technology, Miusskaya Sq., 9, 125047 Moscow, Russia.
Department of Cybernetics of Chemical Technological Processes, Faculty of Digital Technologies and Chemical Engineering, D. Mendeleev University of Chemical Technology, Miusskaya Sq., 9, 125047 Moscow, Russia.
Microorganisms. 2021 Apr 26;9(5):930. doi: 10.3390/microorganisms9050930.
A number of mechanisms have been proposed explaining probiotics and prebiotics benefit human health, in particular, probiotics have a suppression effect on pathogen growth that can be enhanced with the introduction of prebiotics. In vitro models enhanced with computational biology can be useful for selecting a composition with prebiotics from new plant sources with the greatest synergism. Water extracts from burdock root and Jerusalem artichoke tubers were purified by ultrafiltration and activated charcoal and concentrated on a rotary evaporator. Fructans were precipitated with various concentrations of ethanol. 8 VKPM AC-2136 and ATCC 43300 strains were applied to estimate the synbiotic effect. The growth of bifidobacteria and staphylococci in monocultures and cocultures in broths with glucose, commercial prebiotics, as well as isolated fructans were studied. The minimum inhibitory concentrations (MICs) of lactic and acetic acids for the strain were determined. A quantitative model joining the formation of organic acids by probiotics as antagonism factors and the MICs of pathogens (as the measure of their inhibition) was tested in cocultures and showed a high predictive value (R ≥ 0.86). The synbiotic factor obtained from the model was calculated based on the experimental data and obtained constants. Fructans precipitated with 20% ethanol and have the greater synergism against .
已经提出了多种机制来解释益生菌和益生元对人类健康有益的原因，特别是益生菌对病原体生长具有抑制作用，而引入益生元可以增强这种作用。借助计算生物学增强的体外模型有助于从具有最大协同作用的新植物来源中选择含有益生元的组合物。牛蒡根和菊芋块茎的水提取物通过超滤和活性炭进行纯化，并在旋转蒸发器上浓缩。用不同浓度的乙醇沉淀果聚糖。应用8株VKPM AC-2136和ATCC 43300菌株来评估合生元效应。研究了双歧杆菌和葡萄球菌在含有葡萄糖、商业益生元以及分离出的果聚糖的肉汤中的单培养和共培养中的生长情况。测定了乳酸和乙酸对该菌株的最低抑菌浓度（MIC）。在共培养中测试了一个定量模型，该模型将益生菌形成的有机酸作为拮抗因子与病原体的MIC（作为其抑制作用的衡量指标）相结合，显示出较高的预测价值（R≥0.86）。根据实验数据和获得的常数计算出模型得到的合生元因子。用20%乙醇沉淀的果聚糖对……具有更大的协同作用。
