Bosque-Sendra J M, Gámiz-Gracia L, García-Campaña A M
Faculty of Sciences, Department of Analytical Chemistry, University of Granada, Campus Fuentenueva, 18071, Granada, Spain.
Anal Bioanal Chem. 2003 Nov;377(5):863-74. doi: 10.1007/s00216-003-2142-4. Epub 2003 Aug 28.
Flow Injection analysis represents an attractive tool because of its great advantages, such as versatility, speed, high sampling rate and wide applicability in the field of pharmaceutical analysis. However, due to the inherent characteristics of the technique, the choice of the best set of operational and chemical conditions is complicated and the conventional univariate optimisation method present some limitations, mainly due to fact that the interdependence of variables is not considered. In relation to the calibration process, because of the transient character of the signals obtained using FIA manifolds coupled with different detection techniques, different strategies can be used in calibration to solve some problems related to the nature of the signal thereby improving the performance characteristics of the method. This paper offers an overview of different methodologies used in optimisation based on the use of statistically designed experiments and some strategies developed for calibration applied to the analysis of pharmaceuticals.
流动注射分析是一种颇具吸引力的工具，因为它具有诸多显著优势，例如通用性强、速度快、采样率高以及在药物分析领域的广泛适用性。然而，由于该技术的固有特性，选择最佳的操作和化学条件组合较为复杂，并且传统的单变量优化方法存在一些局限性，主要原因是未考虑变量之间的相互依赖性。关于校准过程，由于使用与不同检测技术联用的流动注射分析流路所获得信号具有瞬态特性，在校准中可采用不同策略来解决一些与信号性质相关的问题，从而提高该方法的性能特征。本文概述了基于统计设计实验的优化中所使用的不同方法，以及为应用于药物分析的校准而开发的一些策略。
