Tomiya N, Suzuki T, Awaya J, Mizuno K, Matsubara A, Nakano K, Kurono M
Mie Research Laboratory, Sanwa Kagaku Kenkyusho, Co., Ltd., Japan.
Anal Biochem. 1992 Oct;206(1):98-104. doi: 10.1016/s0003-2697(05)80016-8.
A sensitive and simple high-performance liquid chromatographic method has been developed to determine the concentration of monosaccharides and sugar alcohols in animal tissues. Five neutral monosaccharides (D-glucose, D-galactose, D-mannose, D-fructose, and D-ribose) and three neutral sugar alcohols (myo-inositol, glycerol, and D-sorbitol) predominate in the renal cortices and sciatic nerves of rats. These monosaccharides and sugar alcohols were extracted with distilled water, purified by deproteinization with ethanol, a Sep-Pak C18 cartridge, and columns of Dowex 50W-X8 and Amberlite CG-400, then separated on Ca2+ and Pb2+ cation-exchange columns, eluted with deionized distilled water at 80 degrees C, and detected using integrated pulsed amperometry. About 10 pmol of each sugar was detectable with a signal-to-noise ratio of 10:1. D-Glucose, D-fructose, D-sorbitol, and D-mannose were higher in both the renal and sciatic tissues of diabetic rats than in those of normal animals. D-Ribose and glycerol were higher in the renal cortex of diabetic animals.
已开发出一种灵敏且简便的高效液相色谱法，用于测定动物组织中单核 糖和糖醇的浓度。五种中性单糖（D-葡萄糖、D-半乳糖、D-甘露糖、D-果糖和 D-核糖）和三种中性糖醇（肌醇、甘油和 D-山梨醇）在大鼠肾皮质和坐骨神经 中占主导地位。这些单糖和糖醇用蒸馏水提取，通过用乙醇、Sep-Pak C18 柱 以及 Dowex 50W-X8 和 Amberlite CG-400 柱进行脱蛋白纯化，然后在 Ca2+和 Pb2+阳离子交换柱上分离，于 80℃用去离子蒸馏水洗脱，并使用积分脉冲安培 法进行检测。每种糖约 10 pmol 时可检测到，信噪比为 10:1。糖尿病大鼠的肾 组织和坐骨组织中的 D-葡萄糖、D-果糖、D-山梨醇和 D-甘露糖均高于正常动 物。糖尿病动物肾皮质中的 D-核糖和甘油含量更高。
