Knight J A, Searles D A, Blaylock R C
Laboratory Service, Salt Lake VA Medical Center, UT.
Ann Clin Lab Sci. 1992 Nov-Dec;22(6):417-22.
Prophylactic irradiation of blood and blood components is accepted practice in order to prevent graft-versus-host disease from infused lymphocytes. Irradiation, however, results in increased red cell potassium (K+) loss, along with other possible effects that may affect red cell function and viability. Lipid peroxidation (LP), a process initiated by the production of oxygen free radicals, is increased in red cells in the presence of reactive iron species and various heme moieties. In this report, it is noted that not only is plasma K+ significantly increased following blood irradiation, but LP is also increased compared with paired non-irradiated blood samples. Furthermore, various metal chelators significantly reduce LP in the irradiated samples. These chelators also significantly reduced the rate of cellular K+ loss during the four day 37 degrees C incubation period. This study further suggests that the addition of selected metal chelators may be effective in both irradiated and non-irradiated stored blood by improving the function and viability of transfused erythrocytes.
为防止输注的淋巴细胞引发移植物抗宿主病，对血液及血液成分进行预防性辐照已成为公认的做法。然而，辐照会导致红细胞钾（K+）流失增加，以及其他可能影响红细胞功能和活力的效应。脂质过氧化（LP）是由氧自由基产生引发的过程，在存在活性铁物种和各种血红素部分的情况下，红细胞中的脂质过氧化会增加。在本报告中，注意到血液辐照后不仅血浆K+显著增加，而且与配对的未辐照血液样本相比，LP也增加。此外，各种金属螯合剂可显著降低辐照样本中的LP。这些螯合剂还显著降低了在37℃孵育4天期间细胞K+的流失率。本研究进一步表明，添加选定的金属螯合剂可能通过改善输注红细胞的功能和活力，对辐照和未辐照的储存血液均有效。
