Knight J A, Voorhees R P, Martin L
Laboratory Service, Salt Lake Veteran's Affairs Medical Center, UT.
Ann Clin Lab Sci. 1992 Jul-Aug;22(4):207-13.
Lipid peroxidation, an autocatalytic free radical process, is increased in a wide variety of conditions including various hemolytic anemias and hemoglobin disorders. Increased erythrocyte lipid peroxidation occurs in the presence of iron species and some heme moieties. In this study, greatly reduced concentrations of malondialdehyde (MDA), an indicator of lipid peroxidation, were observed in stored blood anticoagulated with citrate, phosphate, dextrose, and adenine (CPDA-1) upon the addition of deferoxamine mesylate (DM), diethylenetriaminepentaacetic acid (DTPA), and ethylenediaminetetraacetic acid (EDTA) (P less than 0.001). Human transferrin has essentially no effect on lipid peroxidation. Conversely, penicillamine and butylated hydroxytoluene significantly increased lipoperoxide production. Although bathophenanthroline disulfonic acid (BPD) appeared to be very effective in reducing lipid peroxidation, its complex chromatographic patterns which were due to the presence of multiple thiobarbituric acid reactive substances, were difficult to fully evaluate. The addition of appropriate metal chelators to stored blood may be effective in increasing the viability and longevity of transfused red cells.
脂质过氧化是一种自催化自由基过程，在包括各种溶血性贫血和血红蛋白疾病在内的多种情况下都会增加。在铁离子和一些血红素部分存在的情况下，红细胞脂质过氧化会增加。在本研究中，当添加甲磺酸去铁胺（DM）、二乙烯三胺五乙酸（DTPA）和乙二胺四乙酸（EDTA）时，在用枸橼酸盐、磷酸盐、葡萄糖和腺嘌呤（CPDA-1）抗凝的储存血液中，观察到脂质过氧化指标丙二醛（MDA）的浓度大幅降低（P小于0.001）。人转铁蛋白对脂质过氧化基本没有影响。相反，青霉胺和丁基化羟基甲苯显著增加了脂质过氧化物的产生。尽管二氮杂菲二磺酸（BPD）在降低脂质过氧化方面似乎非常有效，但其复杂的色谱图谱（由于存在多种硫代巴比妥酸反应性物质）难以完全评估。向储存血液中添加适当的金属螯合剂可能有效地提高输注红细胞的活力和寿命。
