Kim Jae-Young, Mochizuki Tomoyuki, Akita Keiichi, Jung Han-Sung
Division in Anatomy & Developmental Biology, Department of Oral Biology, Oral Science Research Center, College of Dentistry, BK 21 Project for Medical Sciences, Yonsei University, Seoul, Korea.
Exp Cell Res. 2003 Nov 1;290(2):217-26. doi: 10.1016/s0014-4827(03)00319-7.
The morphogenesis of fungiform papillae occurs in a stereotyped pattern on the dorsal surface of the tongue in mice from embryonic day 12 (E12) to E17. The histological results and ultrastructural observations showed the development of specific structures in the epithelium into fungiform papillae. Prior to the morphological changes, the Bmp-4 and Shh transcripts are expressed in a restricted area on the dorsal surface. These results suggest that the development of fungiform papillae requires an epithelium and mesenchyme interaction during morphogenesis. In order to obtain direct evidence of the epithelium and mesenchyme interaction during tongue papillae morphogenesis, the formation of fungiform papillae was examined after a recombination assay. In order to confirm the epithelium and mesenchyme interactions during the early development of the mouse tongue, a recombination assay was conducted after the recombination assay at E12.5 and E13.5 for 2 days using an in vitro organ culture. From the recombination assay results, the E13.5 epithelial portion of the fungiform papillae could determine the position of the newly formed fungiform papillae with the epithelial signaling molecules. E13.5 was a critical stage for fungiform papillae morphogenesis. Fungiform papillae can be considered to be small epithelial appendages, which are formed via the epithelium and mesenchyme interactions.
菌状乳头的形态发生在小鼠胚胎第12天（E12）至E17天期间，以一种定型模式出现在舌背表面。组织学结果和超微结构观察显示上皮中的特定结构发育成菌状乳头。在形态变化之前，Bmp-4和Shh转录本在背表面的一个受限区域表达。这些结果表明，菌状乳头的发育在形态发生过程中需要上皮和间充质的相互作用。为了获得舌乳头形态发生过程中上皮和间充质相互作用的直接证据，在重组试验后检查了菌状乳头的形成。为了证实小鼠舌早期发育过程中的上皮和间充质相互作用，在E12.5和E13.5进行重组试验后，使用体外器官培养进行了2天的重组试验。从重组试验结果来看，菌状乳头的E13.5上皮部分可以通过上皮信号分子确定新形成的菌状乳头的位置。E13.5是菌状乳头形态发生的关键阶段。菌状乳头可被认为是小上皮附属物，它们是通过上皮和间充质相互作用形成的。
