Jeyendran R S, Van der Ven H H, Zaneveld L J
Department of Obstetrics and Gynecology, Northwestern University Medical School, Prentice Women's Hospital, Chicago, Illinois.
Arch Androl. 1992 Sep-Oct;29(2):105-16. doi: 10.3109/01485019208987714.
The hypoosmotic swelling (HOS) test is a relatively new assay used to evaluate the functional integrity of the sperm's plasma membrane. In fact, more studies have been published on the applicability of the HOS test than any other new sperm indicator. The assay is based on the fact that fluid transport occurs across an intact cell membrane under hypoosmotic conditions until equilibrium is reached. Due to the influx of fluid, the cell will expand and bulge, especially in the tail, and this change can be readily observed with a phase contrast microscope. Earlier studies have yielded some confusion regarding the interpretation of the data. This review is an attempt to clarify and update the usefulness of the HOS test as a tool to evaluate the sperm function.
低渗肿胀（HOS）试验是一种相对较新的检测方法，用于评估精子质膜的功能完整性。事实上，关于HOS试验适用性的研究比任何其他新的精子指标都要多。该检测方法基于这样一个事实，即在低渗条件下，液体通过完整的细胞膜进行转运，直至达到平衡。由于液体的流入，细胞会膨胀并鼓起，尤其是在尾部，这种变化可以用相差显微镜很容易地观察到。早期的研究在数据解释方面产生了一些混淆。这篇综述旨在阐明并更新HOS试验作为评估精子功能工具的实用性。
