Engel S, Petzoldt R
Dermatological Clinic, Medical Faculty (Charité), Humboldt University, Berlin, Germany.
Andrologia. 1994 Nov-Dec;26(6):309-13. doi: 10.1111/j.1439-0272.1994.tb00809.x.
The aim of the present study was to establish a more differentiating indicator of plasma membrane integrity of spermatozoa than the classic version of the hypoosmotic swelling test according to Jeyendraan. Spermatozoa were prepared by density gradient centrifugation (90% Percoll) to select 'fertilization competent' spermatozoa only. After a second washing procedure sufficiently pure sperm cell suspensions were obtained. The volume distributions of these sperm cells were measured with a Coulter Counter at 25 degrees C after adaptation in 300 mosmolar NaCl solution resp. 150 mosmolar NaCl solution for 5 min. These volume distributions showed significantly different patterns for the isotonic and hypotonic stress situation in the simple salt solution. Moreover, the comparison of the response to hypoosmotic stress showed more than four reproducible characteristic patterns, promising well differentiated results for different sperm populations. The new method for the detection of hypoosmotic swelling effect might be a real and valuable functional parameter.
本研究的目的是建立一种比根据杰延德兰经典低渗肿胀试验更能区分精子质膜完整性的指标。通过密度梯度离心法（90% Percoll）制备精子，以仅选择“具有受精能力”的精子。经过第二次洗涤程序后，获得了足够纯净的精子细胞悬液。在300毫渗摩尔氯化钠溶液或150毫渗摩尔氯化钠溶液中适应5分钟后，于25℃用库尔特计数器测量这些精子细胞的体积分布。在简单盐溶液中，等渗和低渗应激情况下，这些体积分布呈现出明显不同的模式。此外，对低渗应激反应的比较显示出四种以上可重复的特征模式，有望为不同精子群体提供区分良好的结果。检测低渗肿胀效应的新方法可能是一个真实且有价值的功能参数。
