Takahashi K, Sakoda R, Mizoguchi S, Yoshino K, Kitao M
Department of Obstetrics/Gynecology, Shimane Medical University, Izumo, Japan.
Arch Androl. 1992 Sep-Oct;29(2):137-45. doi: 10.3109/01485019208987717.
The concentration and motility of human sperm increase is examined following the oral administration of a traditional herbal medicine, Hochuekki-To using the hypoosmotic swelling (HOS) test. After 1 h incubation in Hochuekki-To, the motility of sperm and the results of the HOS test improved, particularly for severely abnormal sperm. For both the original and incubated sperm, the results of the HOS test improved when Hochuekki-To was used in the swim-up medium for the swim-up washing method rather than when Whittingham's T6 solution was used. Hochuekki-To had a favorable, direct effect on human sperm functions, suggesting an effect on the physiological integrity of sperm membrane.
使用低渗肿胀（HOS）试验，研究口服传统草药方剂柴胡桂枝汤后人类精子浓度和活力的变化。在柴胡桂枝汤中孵育1小时后，精子活力及HOS试验结果有所改善，尤其是对严重异常的精子。对于未孵育和孵育后的精子，在用于上浮洗涤法的上浮培养基中使用柴胡桂枝汤时，HOS试验结果均有所改善，而使用惠廷厄姆氏T6溶液时则不然。柴胡桂枝汤对人类精子功能有良好的直接影响，提示其对精子膜的生理完整性有作用。
