Uchida A, Takahashi K, Kitao M
Department of Obstetrics and Gynaecology, Shimane Medical University, Izumo, Japan.
Hum Reprod. 1992 Oct;7(9):1264-7. doi: 10.1093/oxfordjournals.humrep.a137838.
We examined spermatozoa from 135 male patients consulting for infertility to evaluate the usefulness of the hypo-osmotic swelling (HOS) test of sperm fertility for the swim-up washing method. HOS test values significantly improved following the treatment of spermatozoa using the swim-up washing method. There were no differences in the rate of sperm motility between the normal group and the oligozoospermic group following treatment, but the HOS test identified significant differences in swelling rates in both groups before and after treatment. The results of the HOS test following swim-up washing were higher during fertilization attempts for 11 cases of successful intra-uterine insemination (IUI) than during unsuccessful IUI attempts; there were no significant differences between the control group and the successful IUI cases. Moreover, using the lower-normal limits for overall sperm swelling (52%) and g-type sperm swelling (30%) obtained from a normal control group, we found that swelling rates were higher when IUI was successful. These findings indicate that the HOS test is an effective measure of sperm fertilizing function when the swim-up washing method is used for sperm treatment. The score of g-type sperm swelling can be used as a substitute for overall sperm swelling.
我们检查了135名因不育前来咨询的男性患者的精子，以评估精子生育力的低渗肿胀（HOS）试验对上游洗涤法的有效性。使用上游洗涤法处理精子后，HOS试验值显著改善。治疗后，正常组和少精子症组的精子活力率没有差异，但HOS试验发现两组治疗前后的肿胀率存在显著差异。11例宫内人工授精（IUI）成功的受精尝试期间，上游洗涤后的HOS试验结果高于IUI尝试失败期间；对照组和IUI成功病例之间没有显著差异。此外，使用从正常对照组获得的总体精子肿胀（52%）和g型精子肿胀（30%）的较低正常下限，我们发现IUI成功时肿胀率更高。这些发现表明，当使用上游洗涤法处理精子时，HOS试验是评估精子受精功能的有效方法。g型精子肿胀评分可替代总体精子肿胀。
