Hendriks Johannes G E, Ensing Geert T, van Horn Jim R, Lubbers Jaap, van der Mei Henny C, Busscher Henk J
Department of Biomedical Engineering, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
J Control Release. 2003 Oct 30;92(3):369-74. doi: 10.1016/s0168-3659(03)00361-4.
The release profile of antibiotics from antibiotic-loaded bone cement, used to prevent infections in total joint arthroplasty, is neither ideal nor complete. Ultrasound has been used to allow drugs to cross otherwise impermeable barriers. The aim of this study was to establish a possible effect of ultrasound on antibiotic release from bone cements. Samples were made of three commercially available gentamicin-loaded bone cements. Part of the samples was allowed to release gentamicin for 3 weeks before insonation. An insonation device produced an ultrasound field with a time average acoustic intensity of 167 mW/cm2 at a frequency of 46.5 kHz. The samples were exposed to the ultrasound field or not exposed to it as a control. The amount of gentamicin released was measured by fluorescence polarization immunoassay. There was a limited increase of gentamicin release with application of ultrasound in fresh samples but not in the samples that had been allowed to release gentamicin. For fresh samples, a linear regression model showed that this ultrasound effect was statistically significant. The mechanism behind these observations is not clear, but it is suggested that microstreaming or localized temperature rises may be involved.
用于预防全关节置换术中感染的载抗生素骨水泥的抗生素释放情况既不理想也不完整。超声已被用于使药物穿过原本不可渗透的屏障。本研究的目的是确定超声对骨水泥中抗生素释放的可能影响。样本由三种市售的载庆大霉素骨水泥制成。部分样本在超声处理前让庆大霉素释放3周。一个超声装置产生了一个超声场，其时间平均声强为167 mW/cm²，频率为46.5 kHz。样本被暴露于超声场或不暴露于超声场作为对照。通过荧光偏振免疫测定法测量庆大霉素的释放量。在新鲜样本中应用超声时庆大霉素释放量有有限增加，但在已让庆大霉素释放的样本中没有。对于新鲜样本，线性回归模型表明这种超声效应具有统计学意义。这些观察结果背后的机制尚不清楚，但有人认为可能涉及微流或局部温度升高。
