Alva Victor A, Peyton Brent M
Center for Multiphase Environmental Research, Consortium for Extremophile Research, Washington State University, Dana Hall, 118 Spokane Street, Pullman, Washington 99164-2710, USA.
Environ Sci Technol. 2003 Oct 1;37(19):4397-402. doi: 10.1021/es0341844.
Removal of aromatic compounds from alkaline and/or saline industrial wastewater is an environmental concern for industry. In addition, aromatics may be accumulating in soda lakes, unique natural systems, where the fate and toxicity of these contaminants is unknown. To determine the feasibility of aromatic compound biodegradation in saline and alkaline conditions, the effect of pH and salinity on the biodegradation of phenol as a model aromatic waste compound by the haloalkaliphilic bacterium Halomonas campisalis was examined. Phenol was degraded as a source of carbon and energy at pH 8-11 and 0-150 g/L NaCl. Metabolic intermediates catechol, cis,cis-muconate, and (+)-muconolactone were identified, thus indicating that phenol was degraded via the beta-ketoadipate metabolic pathway. Although phenol and catechol were completely degraded in all cases, small amounts of cis,cis-muconate accumulated proportionally to increases in pH. There was no noticeable influence of salinity on cis,cis-muconate accumulation except at 0 g/L NaCl where it was completely degraded. These results indicate that it may be feasible to use haloalkaliphiles forthe treatment of aromatics present in saline and/or alkaline systems. This is the first report of phenol and catechol biodegradation under combined saline and alkaline conditions.
从碱性和/或含盐工业废水中去除芳香族化合物是工业界关注的一个环境问题。此外，芳香族化合物可能正在苏打湖中蓄积，苏打湖是独特的自然系统，这些污染物在其中的归宿和毒性尚不清楚。为了确定在含盐和碱性条件下芳香族化合物生物降解的可行性，研究了pH值和盐度对嗜盐碱细菌坎氏嗜盐单胞菌将苯酚作为典型芳香族废化合物进行生物降解的影响。在pH值为8 - 11和NaCl浓度为0 - 150 g/L的条件下，苯酚作为碳源和能源被降解。鉴定出了代谢中间体儿茶酚、顺，顺-粘康酸和(+)-粘康酸内酯，从而表明苯酚是通过β-酮己二酸代谢途径降解的。尽管在所有情况下苯酚和儿茶酚都被完全降解，但顺，顺-粘康酸的少量积累与pH值的升高成比例。除了在0 g/L NaCl时顺，顺-粘康酸被完全降解外，盐度对其积累没有明显影响。这些结果表明，利用嗜盐碱菌处理含盐和/或碱性系统中存在的芳香族化合物可能是可行的。这是关于在含盐和碱性联合条件下苯酚和儿茶酚生物降解的首次报道。
