Devi Tishu, Sistla Srinivas, Khan Rabiya T, Kailoo Swadha, Bhardwaj Mansavi, Rasool Shafaq
School of Biotechnology, Shri Mata Vaishno Devi University, Katra, J&K, India.
Microbiology and Immunology Department, State University of New York, Stony Brook, New York, United States of America.
PeerJ. 2025 Feb 19;13:e18921. doi: 10.7717/peerj.18921. eCollection 2025.
Extensive and escalating research has been directed towards halozymes derived from halophiles thriving in extreme hypersaline environments, owing to their myriad industrial applications. These extremophiles have evolved various physiological and metabolic adaptations to endure such extremes, enhancing their industrial potential. Being a potential source of lipases, halophiles of extreme niches have emerged as a emerging research area. This interest has been fueled by the recognition that extreme environments serve as rich reservoirs of diverse cold-active alkaliphilic enzymes.
TKW3, isolated from brackish Lake Tso Kar of the Ladakh region, India, produced cold-adapted haloalkaliphilic lipase halozyme. The current study focused on the purification and biochemical characterisation of lipase derived from halophilic bacteria.
The lipase enzyme, purified to homogeneity, exhibited a molecular mass of 28 kDa as confirmed by SDS-PAGE analysis. The purification process yielded a purification fold of 12.01 and a final recovery rate of 29.9%. It demonstrated optimal activity at 30 °C and pH 9. The enzyme was evaluated and demonstrated to exhibit stability over a broad temperature range spanning from 5 °C to 55 °C, as well as a wide pH range of 7.0 to 9.0. Due to its stability across a diverse spectrum of pH values, surfactants, metal ions, and inhibitors, the enzyme appeared to hold significant promise for application within the leather and detergent sectors. Upon undergoing detergent compatibility tests spanning diverse temperature ranges, the lipase showcased compatibility with various commercial detergents, thereby presenting itself as an attractive candidate for inclusion in detergent formulations within the industry.
The lipase from TKW3 exhibits promising attributes, including alkali stability, halophilicity, and a wide spectrum of substrate specificity, rendering it an attractive option for incorporation into detergent formulations within the detergent industry. As far as we are aware, this is the first report on the purification and characterization of lipase enzyme from bacterial halophiles in a Tso Kar brackish lake.
由于其众多的工业应用，针对源自极端高盐环境中嗜盐菌的嗜盐酶的广泛且不断升级的研究已经展开。这些极端微生物已经进化出各种生理和代谢适应性以耐受此类极端环境，从而增强了它们的工业潜力。作为脂肪酶的潜在来源，极端生态位的嗜盐菌已成为一个新兴的研究领域。对极端环境作为多种冷活性嗜碱酶丰富储存库的认识推动了这种兴趣。
从印度拉达克地区的咸水湖措果尔分离出的TKW3产生了冷适应嗜盐嗜碱脂肪酶嗜盐酶。当前的研究集中于嗜盐细菌来源脂肪酶的纯化和生化特性表征。
通过SDS-PAGE分析证实，纯化至同质的脂肪酶分子量为28 kDa。纯化过程的纯化倍数为12.01，最终回收率为29.9%。它在30℃和pH 9时表现出最佳活性。该酶经过评估，证明在5℃至55℃的宽温度范围以及7.0至9.0的宽pH范围内都具有稳定性。由于其在多种pH值、表面活性剂、金属离子和抑制剂范围内的稳定性，该酶在皮革和洗涤剂行业的应用似乎具有很大前景。在进行了不同温度范围的洗涤剂相容性测试后，该脂肪酶与各种商用洗涤剂表现出相容性，因此成为该行业洗涤剂配方中极具吸引力的候选物。
来自TKW3的脂肪酶具有包括碱稳定性、嗜盐性和广泛的底物特异性等有前景的特性，使其成为洗涤剂行业洗涤剂配方中极具吸引力的选择。据我们所知，这是关于从措果尔咸水湖的嗜盐细菌中纯化和表征脂肪酶的首次报道。
