Griffin J F, Nagai S, Buchan G S
Department of Microbiology, University of Otago, Dunedin, New Zealand.
Res Vet Sci. 1991 May;50(3):279-85. doi: 10.1016/0034-5288(91)90124-7.
The use of a Mycobacterium bovis-specific protein, mycobacterial protein bovis 70 (MPB70), was compared with complex, M bovis-derived purified protein derivative (bovine PPD), for its ability to improve the diagnostic precision of in vitro assays for tuberculosis in farmed deer. A combination of lymphocyte transformation and enzyme-linked immunosorbent assay (ELISA) was used to differentiate between specific M bovis reactivity and crossreactivity due to sensitisation with saprophytic mycobacteria such as Mycobacterium avium. In the lymphocyte transformation assay the response of mononuclear cells, from red deer, to MPB70 was found to be more specific, but less sensitive, as an indicator of infection by M bovis when compared with the complex antigen bovine PPD. When used in conjunction with bovine PPD alone, MPB70 was found to increase the specificity of the ELISA in diagnosing animals with disease.
将牛分枝杆菌特异性蛋白——牛分枝杆菌蛋白70（MPB70），与复杂的、源自牛分枝杆菌的纯化蛋白衍生物（牛结核菌素）相比较，评估其提高养殖鹿结核病体外检测诊断准确性的能力。采用淋巴细胞转化试验和酶联免疫吸附测定（ELISA）相结合的方法，区分牛分枝杆菌特异性反应性和因腐生分枝杆菌（如鸟分枝杆菌）致敏引起的交叉反应性。在淋巴细胞转化试验中，发现与复合抗原牛结核菌素相比，马鹿单核细胞对MPB70的反应作为牛分枝杆菌感染指标更具特异性，但敏感性较低。当单独与牛结核菌素联合使用时，发现MPB70可提高ELISA诊断患病动物的特异性。
