Dabrowska-Szponar Maria, Galiński Janusz
Katedra i Zakład Mikrobiologii Lekarskiej Akademii Medycznej w Gdańsku.
Med Dosw Mikrobiol. 2003;55(2):117-24.
Usefulness of PCR--fingerprinting technic using ERIC 1 and ERIC 2 primers for epidemiologic analysis of Group B Streptococci was assessed. 120 strains isolated from various clinical samples were tested. Amplification reactions were carried out in automatic termocycler Poly Gen using two primers ERIC 1 and ERIC 2. Products of the amplification were subjected to horizontal electrophoresis in 1.5% agarose gel. Using primer ERIC 1 six DNA patterns were found. Patterns B and C were the most often encountered, containing 34.2% and 35.0% of strains. Less often pattern D occurred including 12.5% of strains. In case of primer ERIC 2 lower differentiation has been achieved, because only four DNA patterns has been found, three of them occurred with similar frequency 29.2% to 33.3%. Taking into consideration the patterns gained by means of two primers thirteen genotypes have been identified. Dominated two patterns CD and BC, comprising 30.0% and 24.1% Group B Streptococci. More rarely DA and BA occurred (10.8% and 8.3%). Remaining patterns make 0.8% to 6.7% strains. Among reference Group B Streptococci strains belonging to nine serologic types using two primers various genetic patterns were obtained.
评估了使用ERIC 1和ERIC 2引物的PCR指纹技术对B族链球菌进行流行病学分析的实用性。对从各种临床样本中分离出的120株菌株进行了检测。在自动热循环仪Poly Gen中使用两种引物ERIC 1和ERIC 2进行扩增反应。扩增产物在1.5%琼脂糖凝胶中进行水平电泳。使用引物ERIC 1发现了六种DNA模式。模式B和C最常出现，分别包含34.2%和35.0%的菌株。模式D出现的频率较低，占菌株的12.5%。在使用引物ERIC 2的情况下，分化程度较低，因为只发现了四种DNA模式，其中三种出现的频率相似，为29.2%至33.3%。考虑到通过两种引物获得的模式，已鉴定出13种基因型。两种模式CD和BC占主导地位，分别包含30.0%和24.1%的B族链球菌。DA和BA出现的频率较低（分别为10.8%和8.3%）。其余模式占菌株的0.8%至6.7%。在属于九种血清型的B族链球菌参考菌株中，使用两种引物获得了各种遗传模式。
