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一种用于直接鉴定无乳链球菌荚膜型(Ia 至 IX)的多重 PCR 检测方法。

A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae.

机构信息

Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanità, 00161 Rome, Italy.

出版信息

J Microbiol Methods. 2010 Feb;80(2):212-4. doi: 10.1016/j.mimet.2009.11.010. Epub 2009 Dec 1.

DOI:10.1016/j.mimet.2009.11.010
PMID:19958797
Abstract

A multiplex PCR assay for the identification of serotypes Ia to IX of Streptococcus agalactiae was developed. By using a single PCR reaction containing a mix of 19 primers the assay identified each serotype by the analysis of the unique two or three bands pattern on agarose gel.

摘要

建立了一种用于鉴定无乳链球菌血清型 Ia 至 IX 的多重 PCR 检测方法。通过使用包含 19 条引物混合物的单个 PCR 反应,该检测方法通过琼脂糖凝胶上独特的两条或三条带模式分析来鉴定每种血清型。

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