Krzemińska-Jaśkowiak Elzbieta, Michalska Wanda, Cybulski Zefiryn, Chylak Jerzy
Katedra i Zakład Mikrobiologii Lekarskiej AM. im. K. Marcinkowskiego w Poznaniu.
Med Dosw Mikrobiol. 2003;55(2):189-95.
The aim of this study was to determine the incidence of Candida spp. strains in specimens obtained from surgically treated patients as well as to analyze the accompanying bacterial flora, both aerobic and anaerobic. The material came from two groups of patients. In the first group consisting of patients operated for colon and rectum carcinoma, the samples included peritoneal fluid, colon or rectum bioptates, pus, blood, and wound swabs. In the other group, biopsy material and smears from post operation wounds were taken from patients who underwent a surgical treatment of larynx carcinoma. Altogether, 282 various clinical specimens from 165 patients were analysed, and 41 Candida spp. strains were isolated: 39 strains of C. albicans and 2 strains of C. tropicalis. In 20 out of 41 specimens infected with Candida spp. (48.8%) the co-infection with bacterial aerobic flora was found. In 10 cases (24.4%), the fungi were isolated together with aerobic and anaerobic bacterial flora, whereas in 2 specimens (4.9%) the anaerobes and Candida albicans were diagnosed. The remaining 9 samples showed only the presence of Candida spp. (21.9%). From among aerobic bacterial flora Enterococcus spp. strains (n = 17) and Gram negative rods from Enterobacteriaceae family (n = 13) were the most frequently isolated. The bacterial strains of Streptococcus spp. (n = 5), Pseudomonas spp. (n = 3), Staphylococcus spp. and Corynebacterium spp. (2 strains, both) were identified more rarely. Bacteroides spp. were the most frequent members of bacterial anaerobic flora (n = 10). Other isolated anaerobic bacteria were classified as Fusobacterium spp. or Peptostreptococcus spp. (1 strain each). E. coli and Enterococcus spp. strains of aerobic bacterial flora were more frequently isolated together with Candida spp.
(i) Mixed bacterial flora was found to predominate in the clinical material from the patients after surgery. (ii) Candida spp. were most frequently found together with aerobic bacterial flora.
本研究的目的是确定从接受手术治疗的患者标本中分离出的念珠菌属菌株的发生率，并分析其伴生的需氧和厌氧细菌菌群。材料来自两组患者。第一组由接受结肠直肠癌手术的患者组成，样本包括腹腔液、结肠或直肠活检组织、脓液、血液和伤口拭子。另一组则从接受喉癌手术治疗的患者身上获取活检材料和术后伤口涂片。总共分析了来自165名患者的282份不同临床标本，分离出41株念珠菌属菌株：39株白色念珠菌和2株热带念珠菌。在41份感染念珠菌属的标本中，有20份（48.8%）发现伴有需氧细菌菌群的共同感染。在10例（24.4%）中，真菌与需氧和厌氧细菌菌群一起被分离出来，而在2份标本（4.9%）中诊断出厌氧菌和白色念珠菌。其余9份样本仅显示有念珠菌属存在（21.9%）。在需氧细菌菌群中，肠球菌属菌株（n = 17）和肠杆菌科革兰氏阴性杆菌（n = 13）是最常分离出的。链球菌属（n = 5）、假单胞菌属（n = 3）、葡萄球菌属和棒状杆菌属（均为2株）的细菌菌株分离得较少。拟杆菌属是厌氧细菌菌群中最常见的成员（n = 10）。其他分离出的厌氧细菌被归类为梭杆菌属或消化链球菌属（各1株）。需氧细菌菌群中的大肠杆菌和肠球菌属菌株更常与念珠菌属一起被分离出来。
（i）发现手术后患者的临床材料中以混合细菌菌群为主。（ii）念珠菌属最常与需氧细菌菌群一起被发现。
