Yan Yuling, Marriott Gerard
Department of Physiology, University of Wisconsin-Madison, 1300 University Avenue, Madison, WI 53706, USA.
Curr Opin Chem Biol. 2003 Oct;7(5):635-40. doi: 10.1016/j.cbpa.2003.08.017.
Biophotonics techniques, especially those involving fluorescence, are widely used in proteomics to characterize the in vitro interactions between proteins in high-throughput mode. On the other hand, fluorescence-based imaging studies often show that protein activity is regulated through large protein complexes that transiently form at specific sites in the cell. One could therefore argue that a systematic functional analysis of the human proteome requires technologies that are capable of time and spatially resolved, multiplexed analysis of protein interactions within cells.
生物光子学技术，尤其是那些涉及荧光的技术，在蛋白质组学中被广泛用于以高通量模式表征蛋白质之间的体外相互作用。另一方面，基于荧光的成像研究经常表明，蛋白质活性是通过在细胞特定部位瞬时形成的大型蛋白质复合物来调节的。因此，可以认为对人类蛋白质组进行系统的功能分析需要能够对细胞内蛋白质相互作用进行时间和空间分辨的多重分析技术。
