毛细管电泳在肽研究中的应用。

Application of capillary electrophoresis in peptide research.

作者信息

Yildiz E, Grübler G, Hörger S, Zimmermann H, Echner H, Stoeva S, Voelter W

机构信息

Abteilung für Physikalische Biochemie, Physiologisch-chemisches Institut der Universität Tübingen, Germany.

出版信息

Electrophoresis. 1992 Sep-Oct;13(9-10):683-6. doi: 10.1002/elps.11501301145.

DOI:10.1002/elps.11501301145

PMID:1459092

Abstract

Comparison of high performance liquid chromatograms (HPLC) with capillary electrophoresis shows the latter to be superior in many cases owing to rapid separation, high resolution and high sensitivity. This is demonstrated with two examples (i) the isolation of natural peptides from bovine tissue and (ii) characterization of a synthetic peptide mixture with the natural sequence (fragment) of the human immunodeficiency virus (HIV) transmembrane glycoprotein gp 41 env.

摘要

高效液相色谱图（HPLC）与毛细管电泳的比较表明，由于分离速度快、分辨率高和灵敏度高，毛细管电泳在许多情况下更具优势。通过两个例子可以证明这一点：（i）从牛组织中分离天然肽；（ii）对具有人类免疫缺陷病毒（HIV）跨膜糖蛋白gp 41 env天然序列（片段）的合成肽混合物进行表征。