Frosch P J, Kligman A M
Contact Dermatitis. 1976 Dec;2(6):314-24. doi: 10.1111/j.1600-0536.1976.tb03068.x.
A procedure has been designed (the chamber-scarification test), which possesses greatly increased sensitivity for assessing the irritancy of topically applied materials. A forearm test site is criss-cross scarified by drawing a 30-gauge needle over the skin with just enough pressure to cleave the epidermis without drawing blood. The test agent is applied in an aluminum chamber once daily for 3 days. The advantages of the method over conventional patch testing are: enhanced capacity to measure mild irritants, reduced time (3 days versus 10 to 21 days), less effort, less cost, less discomfort for the volunteers and greater reproducibility.
已设计出一种程序（腔室划痕试验），该程序在评估局部应用材料的刺激性方面具有大大提高的灵敏度。通过用30号针头在皮肤上交叉划痕，施加刚好足以划破表皮而不出血的压力，在前臂测试部位进行划痕。测试剂每天在铝制腔室中应用一次，持续3天。该方法相对于传统斑贴试验的优点是：测量轻度刺激物的能力增强、时间缩短（3天对比10至21天）、工作量减少、成本降低、志愿者不适感减轻以及重现性更高。
