Walz D, Bucher K E
Agents Actions. 1982 Oct;12(4):552-65. doi: 10.1007/BF01965942.
Injection of aqueous solutions of topically irritant substances intracutaneously into the skin of juvenile white mice creates a persisting oedema which can be assessed by the number of wrinkles formed upon reefing the skin at the site of injection. An algorithm is presented which transforms the number of wrinkles recorded just before as well as 2.5 h and 6 h after injection into the intensity of the oedematous reaction. The results obtained with a collective of about 10 mice per solution to be tested are reliable and reproducible; they conform to dose-response relations and hence can serve for quantifying local irritancy. The method is used to investigate the effect of pH, and a general model is developed describing the local irritancy of unphysiological pH-values.
将局部刺激性物质的水溶液皮内注射到幼年白鼠的皮肤中，会产生持续的水肿，可通过在注射部位收紧皮肤时形成的皱纹数量来评估。本文提出了一种算法，可将注射前以及注射后2.5小时和6小时记录的皱纹数量转化为水肿反应的强度。对每种待测试溶液使用约10只小鼠的群体所获得的结果是可靠且可重复的；它们符合剂量反应关系，因此可用于量化局部刺激性。该方法用于研究pH值的影响，并建立了一个描述非生理pH值局部刺激性的通用模型。
