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1
Chi enhances heteroduplex DNA levels during recombination.在重组过程中，Chi增强异源双链DNA水平。
Genetics. 1992 Dec;132(4):879-91. doi: 10.1093/genetics/132.4.879.
2
RecBCD Enzyme "Chi Recognition" Mutants Recognize Chi Recombination Hotspots in the Right DNA Context.RecBCD酶“Chi识别”突变体在合适的DNA环境中识别Chi重组热点。
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3
The recA-recBCD dependent recombination pathways of Serratia marcescens and Proteus mirabilis in Escherichia coli: functions of hybrid enzymes and hybrid pathways.粘质沙雷氏菌和奇异变形杆菌在大肠杆菌中的recA-recBCD依赖性重组途径：杂合酶和杂合途径的功能。
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Chain-bias of Escherichia coli Rec-mediated lambda patch recombinants is independent of the orientation of lambda cos.大肠杆菌Rec介导的λ噬菌体斑块重组体的链偏向性与λ噬菌体粘性末端（cos）的方向无关。
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The recombination hot spot chi activates RecBCD recombination by converting Escherichia coli to a recD mutant phenocopy.重组热点chi通过将大肠杆菌转变为recD突变体表型模拟物来激活RecBCD重组。
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6
The RecD subunit of the Escherichia coli RecBCD enzyme inhibits RecA loading, homologous recombination, and DNA repair.大肠杆菌RecBCD酶的RecD亚基会抑制RecA装载、同源重组和DNA修复。
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Orientation of cohesive end site cos determines the active orientation of chi sequence in stimulating recA . recBC-mediated recombination in phage lambda lytic infections.粘性末端位点cos的方向决定了chi序列在噬菌体λ裂解感染中刺激recA.recBC介导的重组时的活性方向。
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8
Bacteriophage P22 Abc2 protein binds to RecC increases the 5' strand nicking activity of RecBCD and together with lambda bet, promotes Chi-independent recombination.噬菌体P22 Abc2蛋白与RecC结合，增强RecBCD的5'链切口活性，并与λ bet一起促进不依赖于Chi序列的重组。
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Chi-stimulated patches are heteroduplex, with recombinant information on the phage lambda r chain.Chi刺激的片段是异源双链体，带有噬菌体λr链上的重组信息。
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10
Homologous pairing in vitro stimulated by the recombination hotspot, Chi.由重组热点Chi体外刺激的同源配对。
Cell. 1991 Jul 26;66(2):361-71. doi: 10.1016/0092-8674(91)90625-9.

引用本文的文献

1
RecBCD enzyme: mechanistic insights from mutants of a complex helicase-nuclease.RecBCD 酶：从复杂解旋酶-核酸酶的突变体中获得的机制见解。
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2
Molecular determinants responsible for recognition of the single-stranded DNA regulatory sequence, χ, by RecBCD enzyme.负责识别 RecBCD 酶的单链 DNA 调控序列 χ 的分子决定因素。
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Physical analyses of E. coli heteroduplex recombination products in vivo: on the prevalence of 5' and 3' patches.大肠杆菌体内异源双链重组产物的物理分析：关于5'和3'片段的普遍性
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Multiple heterologies increase mitotic double-strand break-induced allelic gene conversion tract lengths in yeast.多个异源序列增加酵母中由有丝分裂双链断裂诱导的等位基因转换片段长度。
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Evidence for independent mismatch repair processing on opposite sides of a double-strand break in Saccharomyces cerevisiae.酿酒酵母中双链断裂两侧独立错配修复过程的证据。
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6
Hotspots of homologous recombination.同源重组热点
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7
In vitro reconstitution of homologous recombination reactions.同源重组反应的体外重建。
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8
Biochemistry of homologous recombination in Escherichia coli.大肠杆菌中同源重组的生物化学
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9
The phage lambda orf gene encodes a trans-acting factor that suppresses Escherichia coli recO, recR, and recF mutations for recombination of lambda but not of E. coli.噬菌体λ的orf基因编码一种反式作用因子，该因子可抑制大肠杆菌recO、recR和recF突变，从而促进λ噬菌体的重组，但不促进大肠杆菌的重组。
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10
Primary products of break-induced recombination by Escherichia coli RecE pathway.大肠杆菌RecE途径引发断裂重组的主要产物。
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本文引用的文献

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Effects of DNA heterologies on bacteriophage lambda packaging.DNA异源性对噬菌体λ包装的影响。
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2
Structure of chi hotspots of generalized recombination.广义重组的chi热点结构
Cell. 1981 May;24(2):429-36. doi: 10.1016/0092-8674(81)90333-0.
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Directionality and nonreciprocality of Chi-stimulated recombination in phage lambda.噬菌体λ中Chi刺激重组的方向性和非互惠性
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A new class of Escherichia coli recBC mutants: implications for the role of RecBC enzyme in homologous recombination.一类新型大肠杆菌recBC突变体：RecBC酶在同源重组中的作用探讨
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Escherichia coli RecBC pseudorevertants lacking chi recombinational hotspot activity.缺乏契合成重组热点活性的大肠杆菌RecBC假回复突变体。
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Homologous association of chromosomal DNA during yeast meiosis.
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Insertions, deletions and mismatches in heteroduplex DNA made by recA protein.RecA蛋白形成的异源双链DNA中的插入、缺失和错配。
Cell. 1983 Dec;35(2 Pt 1):511-20. doi: 10.1016/0092-8674(83)90185-x.
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Clustering of mutations inactivating a Chi recombinational hotspot.
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9
Evidence for inclusion of regions of nonhomology in heteroduplex products of bacteriophage lambda recombination.噬菌体λ重组异源双链产物中包含非同源区域的证据。
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Recombination in bacteriophage lambda. I. Mutants deficient in general recombination.噬菌体λ中的重组。I. 一般重组缺陷型突变体
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在重组过程中，Chi增强异源双链DNA水平。

Chi enhances heteroduplex DNA levels during recombination.

作者信息

Holbeck S L, Smith G R

机构信息

Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

出版信息

Genetics. 1992 Dec;132(4):879-91. doi: 10.1093/genetics/132.4.879.

DOI:10.1093/genetics/132.4.879
PMID:1459441
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1205245/
Abstract

The major pathway of homologous recombination in Escherichia coli, the RecBCD pathway, is stimulated by Chi sites. To determine whether Chi enhances an early or late step in recombination, we measured formation of heteroduplex DNA (hDNA) in extracts of lambda-infected E. coli. Chi elevated hDNA levels in these extracts, supporting a role for Chi early (before hDNA formation) in recombination. RecA protein and RecBCD enzyme were both necessary for detection of hDNA, indicating that they, too, act early. Analysis of a panel of recBCD mutants indicated that Chi-nicking activity was needed for Chi's stimulation of hDNA formation. These results support a previously proposed model of recombination. Further results suggested that RecBCD enzyme has an additional role late in recombination.

摘要

大肠杆菌中同源重组的主要途径，即RecBCD途径，受到Chi位点的刺激。为了确定Chi是增强重组的早期步骤还是晚期步骤，我们测量了λ感染的大肠杆菌提取物中异源双链DNA（hDNA）的形成。Chi提高了这些提取物中的hDNA水平，支持了Chi在重组早期（hDNA形成之前）发挥作用的观点。RecA蛋白和RecBCD酶对于检测hDNA都是必需的，这表明它们也在早期起作用。对一组recBCD突变体的分析表明，Chi切割活性是Chi刺激hDNA形成所必需的。这些结果支持了先前提出的重组模型。进一步的结果表明，RecBCD酶在重组后期还有额外的作用。