Colsch Benoit, Afonso Carlos, Popa Iuliana, Portoukalian Jacques, Fournier Françoise, Tabet Jean-Claude, Baumann Nicole
Institut National de la Santé et de la Recherche Médicale U495, Laboratoire de Neurochimie, Hopital de la Salpetriere, 75651 Paris 13, France.
J Lipid Res. 2004 Feb;45(2):281-6. doi: 10.1194/jlr.M300331-JLR200. Epub 2003 Nov 1.
Sphingoglycolipids (SGLs) are cell membrane constituents. As the ceramide structure influences the biological properties of the SGL, we characterized by electrospray ionization tandem mass spectrometry the molecular species of ceramides present in SGL of mouse brain. We report here for the first time the presence in mammalian brain of sphingadienine (d18:2). Sphingenine (d18:1) is present in all SGL species, in contrast to eicosasphingenine (d20:1), which is a constituent of only gangliosides. Sphingadienine is present in galactosylceramide and sulfatides. Free ceramides contain the three types of bases. Thus, there could be two separate pools of free ceramides (d18:1, d18:2 and d20:1, d18:1) as precursors of complex SGL.
鞘糖脂（SGLs）是细胞膜的组成成分。由于神经酰胺结构会影响鞘糖脂的生物学特性，我们通过电喷雾电离串联质谱法对小鼠脑鞘糖脂中存在的神经酰胺分子种类进行了表征。我们首次在此报告了鞘氨二烯（d18:2）在哺乳动物脑中的存在情况。鞘氨醇（d18:1）存在于所有鞘糖脂种类中，与之形成对比的是，二十碳鞘氨醇（d20:1）仅为神经节苷脂的组成成分。鞘氨二烯存在于半乳糖神经酰胺和硫脂中。游离神经酰胺包含这三种类型的碱基。因此，可能存在两个独立的游离神经酰胺池（d18:1、d18:2和d20:1、d18:1）作为复合鞘糖脂的前体。
