Risco Ester, Ghia Felipe, Vila Roser, Iglesias José, Alvarez Elida, Cañigueral Salvador
Unitat de Farmacologia i Farmacognòsia, Facultat de Farmàcia, Universitat de Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain.
Planta Med. 2003 Sep;69(9):785-94. doi: 10.1055/s-2003-43208.
The immunomodulatory activity of the latex from Croton lechleri (sangre de drago) was determined by in vitro assays. Classical (CP) and alternative (AP) complement pathways activities were determined in human serum. Intracellular generation of reactive oxygen species (ROS) by human polymorphonuclear leukocytes (PMNs) and monocytes, and phagocytosis of opsonised fluorescent microspheres were measured by flow cytometry. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH). Activity on proliferation of murine lymphocytes was also investigated. In addition, anti-inflammatory activity was assayed in vivo by carrageenan-induced rat paw oedema test. Some of the activities were compared with those of the isolated alkaloid taspine. Sangre de drago from Croton lechleri showed immunomodulatory activity. It exhibited a potent inhibitory activity on CP and AP of complement system and inhibited the proliferation of activated T-cells. The latex showed free radical scavenging capacity. Depending on the concentration, it showed antioxidant or prooxidant properties, and stimulated or inhibited the phagocytosis. Moreover, the latex has strong anti-inflammatory activity when administered i. p. Taspine cannot be considered the main responsible for these activities, and other constituents, probably proanthocyanidins, should be also involved.
通过体外试验测定了巴豆属植物（龙血树）乳胶的免疫调节活性。在人血清中测定经典（CP）和替代（AP）补体途径活性。通过流式细胞术测量人多形核白细胞（PMN）和单核细胞的活性氧（ROS）的细胞内产生，以及调理荧光微球的吞噬作用。使用1,1-二苯基-2-苦基肼（DPPH）评估自由基清除活性。还研究了对小鼠淋巴细胞增殖的活性。此外，通过角叉菜胶诱导的大鼠足肿胀试验在体内测定抗炎活性。将其中一些活性与分离出的生物碱塔斯品的活性进行了比较。巴豆属植物的龙血树显示出免疫调节活性。它对补体系统的CP和AP表现出强大的抑制活性，并抑制活化T细胞的增殖。乳胶显示出自由基清除能力。根据浓度的不同，它表现出抗氧化或促氧化特性，并刺激或抑制吞噬作用。此外，腹腔注射时乳胶具有很强的抗炎活性。塔斯品不能被认为是这些活性的主要原因，其他成分，可能是原花青素，也应该参与其中。
