Vecherov A E, Aianot P K, Timina A M, Lisitsin V V
Vopr Virusol. 2003 Sep-Oct;48(5):46-9.
A possibility of using the amplification of gene HN fragment in combination with nucleotide cDNA sequencing for the purpose of identification and strain differentiation of bovine parainfluenza-3 virus was demonstrated. A comparative analysis of the primary structure in the studied HN gene fragment revealed 2 genetic groups among the investigated virus' strains and isolates. Group 1 is made up of Northern American viral strains and of Russian isolates, whose primary structure has a high level of homology to the primary SF-4/32 strain structure; group 2 comprises the virus' Russian isolates with a high level of homology to the mentioned strains to Japanese strains' sequences. The biggest differences between the studied strains and the viral isolates amounted to around 8%, when the nucleotide sequences were compared, and to around 4%, when the corresponding amino-acid sequences were compared.
已证实，结合基因HN片段扩增与核苷酸cDNA测序用于牛副流感3型病毒的鉴定和毒株分化是可行的。对所研究的HN基因片段一级结构的比较分析显示，在所调查的病毒毒株和分离株中有2个遗传组。第1组由北美病毒毒株和俄罗斯分离株组成，其一级结构与SF-4/32原始毒株结构具有高度同源性；第2组包括与上述毒株的日本毒株序列具有高度同源性的俄罗斯病毒分离株。比较核苷酸序列时，所研究的毒株与病毒分离株之间的最大差异约为8%；比较相应氨基酸序列时，最大差异约为4%。
