Horwood Paul Francis, Gravel Jennifer Lillian, Mahony Timothy John
Department of Primary Industries and Fisheries, Ritchie Building 64A, Level 3, C Wing, Research Road, St Lucia, QLD 4072, Australia.
J Gen Virol. 2008 Jul;89(Pt 7):1643-1648. doi: 10.1099/vir.0.2008/000026-0.
The partial gene sequencing of the matrix (M) protein from seven clinical isolates of bovine parainfluenza virus type 3 (BPIV-3), and the complete sequencing of a representative isolate (Q5592) was completed in this study. Nucleotide sequence analysis was initiated because of the failure of in-house BPIV-3 RT-PCR methods to yield expected products for four of the isolates. Phylogenetic reconstructions based on the nucleotide sequences for the M-protein and the entire genome, using all of the available BPIV-3 nucleotide sequences, demonstrated that there were two distinct BPIV-3 genotypes (BPIV-3a and BPIV-3b). These newly identified genotypes have implications for the development of BPIV-3 molecular detection methods and may also impact on BPIV-3 vaccine formulations.
本研究完成了7株牛副流感3型病毒（BPIV-3）临床分离株基质（M）蛋白的部分基因测序，并对一株代表性分离株（Q5592）进行了全序列测定。由于内部BPIV-3 RT-PCR方法未能从4株分离株中获得预期产物，因此开展了核苷酸序列分析。利用所有可用的BPIV-3核苷酸序列，基于M蛋白和全基因组的核苷酸序列进行系统发育重建，结果表明存在两种不同的BPIV-3基因型（BPIV-3a和BPIV-3b）。这些新鉴定的基因型对BPIV-3分子检测方法的开发具有重要意义，也可能影响BPIV-3疫苗配方。
