Aix Marseille Université, CNRS, Laboratoire de Chimie Bactérienne (UMR7283), IMM, IM2B, 13402 Marseille, France.
Molecules. 2023 Oct 20;28(20):7195. doi: 10.3390/molecules28207195.
In prokaryotes, the role of Mo/W enzymes in physiology and bioenergetics is widely recognized. It is worth noting that the most diverse family of Mo/W enzymes is exclusive to prokaryotes, with the probable existence of several of them from the earliest forms of life on Earth. The structural organization of these enzymes, which often include additional redox centers, is as diverse as ever, as is their cellular localization. The most notable observation is the involvement of dedicated chaperones assisting with the assembly and acquisition of the metal centers, including Mo/W-bisPGD, one of the largest organic cofactors in nature. This review seeks to provide a new understanding and a unified model of Mo/W enzyme maturation.
在原核生物中，Mo/W 酶在生理学和生物能量学中的作用已得到广泛认可。值得注意的是，Mo/W 酶中最多样化的家族是原核生物所特有的，可能存在于地球上最早的生命形式中。这些酶的结构组织通常包括其他氧化还原中心，其细胞定位也多种多样。最值得注意的是，专门的伴侣蛋白参与了金属中心的组装和获取，包括 Mo/W-bisPGD，这是自然界中最大的有机辅因子之一。本综述旨在提供对 Mo/W 酶成熟机制的新理解和统一模型。
