Roberta Tiozzo, Federico Magagna, Federica Boraldi, Antonietta Croce Maria, Sergio Bortolini, Ugo Consolo
Department of Biomedical Sciences, Section of General Pathology, University of Modena and Reggio Emilia, Via G. Campi 287, 4100 Modena, Italy.
Toxicol In Vitro. 2003 Oct-Dec;17(5-6):657-62. doi: 10.1016/s0887-2333(03)00107-3.
The aim of this study was to assess the cytotoxicity of tow types of impression dental materials: polyethers (Impregum Penta, Permadyne Penta Heavy and Light) and vinyl polysiloxanes (Elite Mono Tray, Medium, Low viscosity and Elite H-D Putty). Their cytotoxic effects were studied by indirect and direct tests. The indirect tests were performed by incubating impression materials in serum free cell culture medium to prepare the soluble extracts. Balb/c 3T3 cells were incubated with extract dilutions (25, 50, 75 and 100%) for 24 h. The extracts of polyether materials caused a decrease of cellular viability, evaluated by light microscopy, by cell counting and by MTT test. The extracts of vinyl polysiloxanes materials induced a slight effect on cellular number and viability. The direct tests were performed by placing the impression materials in the centre of Petri dishes while Balb/c 3T3 were settling. The cellular proliferation was drastically reduced by polyethers and it was unaffected by the presence of vinyl polysiloxanes. These results show that: (a) the polyether materials are more toxic than vinyl polysiloxanes in our experimental conditions, (b) the impression materials are cytotoxic to the same degree in all assay methods.
聚醚类（Impregum Penta、Permadyne Penta Heavy和Light）和乙烯基聚硅氧烷类（Elite Mono Tray、Medium、Low viscosity和Elite H-D Putty）。通过间接和直接试验研究了它们的细胞毒性作用。间接试验通过将印模材料在无血清细胞培养基中孵育以制备可溶性提取物来进行。将Balb/c 3T3细胞与提取物稀释液（25%、50%、75%和100%）孵育24小时。通过光学显微镜、细胞计数和MTT试验评估，聚醚材料的提取物导致细胞活力下降。乙烯基聚硅氧烷材料的提取物对细胞数量和活力有轻微影响。直接试验通过在Balb/c 3T3细胞沉降时将印模材料放置在培养皿中央来进行。聚醚类材料使细胞增殖急剧减少，而乙烯基聚硅氧烷的存在对其没有影响。这些结果表明：（a）在我们的实验条件下，聚醚材料比乙烯基聚硅氧烷毒性更大；（b）在所有检测方法中，印模材料的细胞毒性程度相同。
