Kwon Jae-Sung, Piao Yin-Zhu, Cho Sun-A, Yang Song-Yi, Kim Ji Hoon, An Susun, Kim Kwang-Mahn
BK21 PLUS Project, Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Korea.
Safety Research Team/Skin Research Division, Amore-Pacific R&D Center, 1920 Yonggu-daero, Giheung-gu, Yongin-si, Gyeonggi-do 446-729, Korea.
Materials (Basel). 2015 Oct 29;8(11):7269-7277. doi: 10.3390/ma8115372.
Dental luting cements are commonly used in dentistry for cementation of prosthetic restoration. Many previous studies focused on the measurement of the cell viability as the method of cytotoxicity evaluation during biocompatibility study for the material. In this study, the biocompatibility of various dental luting cements were evaluated using the new method of cytokine release measurement in order to better simulate inflammatory reactions in animal or clinical model using two different oral cells; immortalized human gingival fibroblast and immortalized human oral keratinocytes. Cells were exposed to extractions of various commercially available dental luting cements for different durations. Cytokines of IL-1α and IL-8 were measured from the supernatants of the cells and the results were then compared to the conventional MTT viability test. The result from the conventional cell viability study showed a relatively simple and straight forward indication that only one of the dental luting cements tested in this study was cytotoxic with increasing duration of exposure for both cells. Meanwhile, the result from the cytokine measurement study was much more complex at the time point they were measured, type of cells used for the study and the type of cytokines measured, all of which influenced the interpretation of the results. Hence, the better understanding of the cytokine release would be required for the application in biocompatibility evaluation.
牙科粘结水门汀在牙科领域常用于修复体的粘结。在材料生物相容性研究中，许多先前的研究将细胞活力的测定作为细胞毒性评估方法。在本研究中，为了更好地模拟动物或临床模型中的炎症反应，使用两种不同的口腔细胞（永生化人牙龈成纤维细胞和永生化人口腔角质形成细胞），通过测量细胞因子释放的新方法评估了各种牙科粘结水门汀的生物相容性。将细胞暴露于各种市售牙科粘结水门汀的提取物中不同时长。从细胞上清液中测量白细胞介素-1α（IL-1α）和白细胞介素-8（IL-8）的细胞因子，并将结果与传统的MTT活力试验进行比较。传统细胞活力研究的结果显示出相对简单直接的迹象，即本研究中测试的牙科粘结水门汀中只有一种在两种细胞的暴露时间增加时具有细胞毒性。同时，细胞因子测量研究的结果在测量时间点、用于研究的细胞类型和测量的细胞因子类型方面要复杂得多，所有这些都影响了结果的解释。因此，在生物相容性评估中的应用需要对细胞因子释放有更好的理解。
