Düzgün Nurşen, Ayaşlioğlu Ergin, Tutkak Hüseyin, Aydintuğ Olcay T
Department of Internal Medicine, Clinical Immunology, and Rheumatology, Medical School of Ankara University, Ankara, Turkey.
Rheumatol Int. 2005 Jan;25(1):1-5. doi: 10.1007/s00296-003-0400-6. Epub 2003 Nov 5.
Serum levels of proinflammatory cytokines, interleukin-1 beta (IL-1beta), tumor necrosis factor alpha, (TNF-alpha), and their inhibitors, IL-1 receptor antagonist (IL-1ra) and soluble TNF receptor 1 (sTNFR1), were determined by enzyme-linked immunosorbent assay in 104 patients with Behçet's disease (65 active, 39 inactive) and 40 healthy controls. The levels of IL-1beta and IL-1ra were significantly higher in both active and inactive patients than in control subjects (P<0.01 and P< 0.01, respectively). The concentrations of TNF-alpha and sTNFR1 were found to be higher in active patients than in controls (P< 0.01 and P< 0.001, respectively). There were no significant differences in the serum levels of these cytokines and their inhibitors between active and inactive patients. Significant increases in mean C-reactive protein level and erythrocyte sedimentation rate were found in patients with active vs inactive disease (P< 0.001 and P< 0.05, respectively). C-reactive protein values correlated with erythrocyte sedimentation rate but not with cytokines or their inhibitors. Our conclusion is that elevated serum TNF-alpha and sTNFR1 seem to be important inflammatory mediators in Behcet's disease. The statistically significant increase in these levels may arise from the severity of inflammation in the tissue or organ involved.
通过酶联免疫吸附测定法，测定了104例白塞病患者（65例活动期、39例非活动期）及40名健康对照者血清中促炎细胞因子白细胞介素-1β（IL-1β）、肿瘤坏死因子α（TNF-α）及其抑制剂白细胞介素-1受体拮抗剂（IL-1ra）和可溶性肿瘤坏死因子受体1（sTNFR1）的水平。活动期和非活动期患者的IL-1β和IL-1ra水平均显著高于对照者（分别为P<0.01和P<0.01）。发现活动期患者的TNF-α和sTNFR1浓度高于对照者（分别为P<0.01和P<0.001）。活动期和非活动期患者这些细胞因子及其抑制剂的血清水平无显著差异。活动期与非活动期患者相比，平均C反应蛋白水平和红细胞沉降率显著升高（分别为P<0.001和P<0.05）。C反应蛋白值与红细胞沉降率相关，但与细胞因子或其抑制剂无关。我们的结论是，血清TNF-α和sTNFR1升高似乎是白塞病重要的炎症介质。这些水平的统计学显著升高可能源于受累组织或器官炎症的严重程度。
