Staniszewska Magdalena, Gamian Andrzej
Zakład Immunologii Chorób Zakaźnych Instytutu Immunologii i Terapii Doświadczalnej PAN.
Postepy Hig Med Dosw. 2003;57(4):359-68.
The advanced glycation end-products (AGEs) are the compounds formed and accumulated in the organism. There is heterogenous group of the glycation products, which have great variety of structural and functional properties. The glycation end-products can be used as markers of the glycation process. Because of the great heterogeneity of the AGEs there is no specific test for their adequate measurement. In this paper there are presented the chromatographic, colorimetric, spectroscopic, mass-spectrometric and serological methods, which are used for determination of AGEs in biological samples. New procedures for the preparation of the model AGEs have been also described. Special attention has been paid to the immunochemical tests. For further assays the model AGE antigens have to be prepared.
晚期糖基化终末产物（AGEs）是在生物体内形成并积累的化合物。糖基化产物种类繁多，具有各种各样的结构和功能特性。糖基化终末产物可作为糖基化过程的标志物。由于AGEs具有高度的异质性，因此没有用于其充分测量的特异性检测方法。本文介绍了用于测定生物样品中AGEs的色谱法、比色法、光谱法、质谱法和血清学方法。还描述了制备模型AGEs的新程序。特别关注了免疫化学检测。为了进一步检测，必须制备模型AGE抗原。
