Burns J C, Matsubara T, Lozinski G, Yee J K, Friedmann T, Washabaugh C H, Tsonis P A
Department of Pediatrics, UCSD School of Medicine, La Jolla 92093-0609.
Dev Biol. 1994 Sep;165(1):285-9. doi: 10.1006/dbio.1994.1253.
Limb regeneration is a unique developmental phenomenon restricted to certain urodeles in which limb cells dedifferentiate and produce the blastema and then redifferentiate into the tissues that compose the missing part. Genetic modification of the blastema cells would greatly facilitate understanding the programmed gene expression that results in the reconstitution of the limb. To test whether pantropic retroviral vectors pseudotyped with the vesicular stomatitis virus G glycoprotein could mediate gene transfer into blastema cells, we infected a stable newt limb cell line and demonstrated integration and expression of the provirus. Thus, pantropic retroviral vectors offer a new tool for the study of limb regeneration and other developmental phenomena in amphibia.
肢体再生是一种独特的发育现象，仅限于某些有尾两栖动物，在这些动物中，肢体细胞去分化并产生芽基，然后再分化成构成缺失部分的组织。对芽基细胞进行基因改造将极大地有助于理解导致肢体重建的程序性基因表达。为了测试用水泡性口炎病毒G糖蛋白假型化的泛嗜性逆转录病毒载体是否能介导基因转移到芽基细胞中，我们感染了一个稳定的蝾螈肢体细胞系，并证明了前病毒的整合和表达。因此，泛嗜性逆转录病毒载体为研究两栖动物的肢体再生和其他发育现象提供了一种新工具。
