Verweij Jaco J, Laeijendecker Daphne, Brienen Eric A T, van Lieshout Lisette, Polderman Anton M
Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands.
J Clin Microbiol. 2003 Nov;41(11):5041-5. doi: 10.1128/JCM.41.11.5041-5045.2003.
Classically, detection of Entamoeba histolytica is performed by microscopic examination for characteristic cysts and/or trophozoites in fecal preparations. Differentiation of E. histolytica cysts and those of nonpathogenic amoebic species is made on the basis of the appearance and the size of the cysts. However, by classical means objective tools for confirmation and quality control do not exist. Therefore, a reverse line blot hybridization assay was developed to detect a variety of Entamoeba species and genetic variants known to infect humans. The assay was performed after amplification with general Entamoeba-specific primers. The assay could identify four genetic variants of Entamoeba polecki-like cysts as well as E. histolytica, Entamoeba dispar, Entamoeba hartmanni, Entamoeba moshkovskii and Entamoeba coli and even mixed infections in a range of controls and fecal samples. This technique can be used as an additional standard for diagnosis, epidemiology, and quality control for amoebic infections.
传统上，溶组织内阿米巴的检测是通过显微镜检查粪便标本中的特征性包囊和/或滋养体来进行的。溶组织内阿米巴包囊与非致病性阿米巴物种包囊的区分是基于包囊的外观和大小。然而，传统方法不存在用于确认和质量控制的客观工具。因此，开发了一种反向线印迹杂交检测方法，以检测已知感染人类的多种内阿米巴物种和基因变体。该检测在使用通用的内阿米巴特异性引物扩增后进行。该检测能够识别似波列基内阿米巴包囊的四种基因变体以及溶组织内阿米巴、迪斯帕内阿米巴、哈氏内阿米巴、莫氏内阿米巴和结肠内阿米巴，甚至在一系列对照和粪便样本中识别混合感染。这项技术可作为阿米巴感染诊断、流行病学和质量控制的附加标准。
