Oakeshott J G
Aust J Biol Sci. 1976 Oct;29(4):365-73. doi: 10.1071/bi9760365.
This paper describes substrate specificities, developmental changes in activity, pH profiles, and heat stabilities of isozymes produced by four Adh genotypes in D. melanogaster. No differences are found in the substrate specificities of isozymes from the different genotypes but studies of the other three properties reveal significant differences between the isozymes. Thus relatively low activities are found among extracts of AdhF Adhn2 larvae and among extracts of AdhF AdhF adults aged 44 days. Also AdhF AdhS and AdhS AdhS extracts have relatively high activities at pH 6-5, and AdhF Adhn2 extracts have relatively low activities at pH values above 10-0. Finally, extracts of AdhF AdhF and AdhF AdhS are more stable at 40 degrees C than are those of AdhS AdhS and AdhF Adhn2.
本文描述了黑腹果蝇中四种乙醇脱氢酶（Adh）基因型所产生的同工酶的底物特异性、活性的发育变化、pH 谱以及热稳定性。不同基因型的同工酶在底物特异性上未发现差异，但对其他三个特性的研究揭示了同工酶之间存在显著差异。因此，在 AdhF Adhn2 幼虫的提取物以及 44 日龄的 AdhF AdhF 成虫的提取物中发现活性相对较低。此外，AdhF AdhS 和 AdhS AdhS 提取物在 pH 6.5 时具有相对较高的活性，而 AdhF Adhn2 提取物在 pH 值高于 10.0 时具有相对较低的活性。最后，AdhF AdhF 和 AdhF AdhS 的提取物在 40℃ 时比 AdhS AdhS 和 AdhF Adhn2 的提取物更稳定。
