Dang Cheng-Xue, Han Yue, Qin Zhao-Yin, Wang Ya-Jun
Department of Tumor Surgery, First Hospital, Xi'an Jiaotong University, Xi'an 710061, China.
Hepatobiliary Pancreat Dis Int. 2002 May;1(2):302-5.
To study the clinical significance and effect of p21, p53 protein as well as proliferating cell nuclear antigen (PCNA) on the occurrence and development of pancreatic carcinoma.
p21, p53 protein and PCNA expressions were detected in specimens from 30 patients with pancreatic carcinoma and 3 samples of normal pancreatic tissue by immunohistochemistry. The data were analyzed together with clinical findings.
The positive expression rates of p21 and p53 proteins were 75.0% and 57.3% respectively in pancreatic carcinoma, which were significantly different from those in the normal tissue (P<0.05). p21 and p53 proteins were positively correlated (P<0.05). The positive expression of PCNA was 43.33%+/-17.99%, that was significantly higher than that in the normal pancreatic tissue (P<0.05). The expression of PCNA was correlated with the histological grade (P<0.05). The positive expression rate was consistent with the exacerbation of cancer. The expression was also correlated significantly with prognosis and p53 expression (P<0.05).
The occurrence and development of pancreatic cancer are the result of associated function for many oncogenes and antioncogenes. PCNA may be helpful to identify malignant degree and prognosis of pancreatic cancer.
研究p21、p53蛋白以及增殖细胞核抗原（PCNA）在胰腺癌发生发展中的临床意义及作用。
采用免疫组织化学法检测30例胰腺癌患者标本及3例正常胰腺组织标本中p21、p53蛋白和PCNA的表达情况，并结合临床资料进行分析。
胰腺癌中p21和p53蛋白的阳性表达率分别为75.0%和57.3%，与正常组织相比差异有统计学意义（P<0.05）。p21和p53蛋白呈正相关（P<0.05）。PCNA的阳性表达率为43.33%±17.99%，显著高于正常胰腺组织（P<0.05）。PCNA的表达与组织学分级相关（P<0.05），阳性表达率随癌症进展而升高。其表达还与预后及p53表达显著相关（P<0.05）。
胰腺癌的发生发展是多种癌基因和抑癌基因共同作用的结果。PCNA可能有助于判断胰腺癌恶性程度及预后。
