Geyer Hildegard, Wuhrer Manfred, Kurokawa Tomofumi, Geyer Rudolf
Faculty of Medicine, Institute of Biochemistry, University of Giessen, Friedrichstrasse 24, Giessen D-35392, Germany.
Micron. 2004;35(1-2):105-6. doi: 10.1016/j.micron.2003.10.033.
Keyhole limpet hemocyanin (KLH) is known to share carbohydrate epitopes with Schistosoma mansoni. In order to define the structural basis for the observed serological cross-reactivity, KLH glycans were released either by enzyme treatment or by hydrazinolysis and probed with a rabbit hyperimmune serum directed against S. mansoni egg antigen. Both major, non-reacting oligosaccharide species as well as the minor compounds recognized were isolated by two-dimensional high performance liquid chromatography and in part by lectin affinity chromatography, and characterized by mass spectrometry. The results revealed that KLH carries predominantly high mannose-type glycans as well as short sugar chains. As a characteristic feature, a number of the latter glycans contained a Gal(beta1-6)Man-unit, which has not yet been found in glycoprotein-N-glycans. Oligosaccharides cross-reacting with schistosomal glycans comprised a terminal Fuc(alpha1-3)GalNAc-motif, which appears to represent the main carbohydrate epitope mediating cross-reactivity of KLH with glycoconjugates from S. mansoni.
已知匙孔血蓝蛋白（KLH）与曼氏血吸虫具有共同的碳水化合物表位。为了确定所观察到的血清学交叉反应性的结构基础，通过酶处理或肼解释放KLH聚糖，并用针对曼氏血吸虫卵抗原的兔超免疫血清进行检测。通过二维高效液相色谱法，部分通过凝集素亲和色谱法，分离出主要的无反应性寡糖种类以及识别出的次要化合物，并通过质谱进行表征。结果显示，KLH主要携带高甘露糖型聚糖以及短糖链。作为一个特征，许多后者的聚糖含有一个Gal(β1-6)Man单元，该单元尚未在糖蛋白N-聚糖中发现。与血吸虫聚糖发生交叉反应的寡糖包含一个末端Fuc(α1-3)GalNAc基序，这似乎代表了介导KLH与曼氏血吸虫糖缀合物交叉反应性的主要碳水化合物表位。
