短时间尿液冻存对建立的实时荧光 PCR 检测日本血吸虫敏感性的影响。
Impact of short-time urine freezing on the sensitivity of an established Schistosoma real-time PCR assay.
机构信息
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands.
出版信息
Am J Trop Med Hyg. 2014 Jun;90(6):1153-5. doi: 10.4269/ajtmh.14-0005. Epub 2014 Apr 7.
Abstract
Urogenital schistosomiaisis is a serious public health problem in sub-Saharan Africa. In this study, we have updated an established real-time polymerase chain reaction (PCR) routinely used in our laboratory. Schistosoma genus-specific real-time PCR was performed on DNA isolated from 85 urine samples and pellets obtained after centrifugation without and after frozen storage. The results revealed that concentration by centrifugation of the urine samples and freezing of the samples before extracting DNA improves the sensitivity of the PCR.
摘要
泌尿生殖系统血吸虫病是撒哈拉以南非洲的一个严重公共卫生问题。在这项研究中，我们更新了实验室常规使用的成熟实时聚合酶链反应（PCR）。对从 85 份尿液样本和离心后未冷冻及冷冻储存后的沉淀中提取的 DNA 进行了血吸虫属实时 PCR。结果表明，离心浓缩尿液样本和在提取 DNA 前冷冻样本可提高 PCR 的灵敏度。
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