Uesugi Takehiko, Toda Toshiya, Okuhira Takenori, Chen Jui-Tung
Research & Development Laboratory, Fujicco Co., Ltd., Kobe, Japan.
Endocr J. 2003 Oct;50(5):613-9. doi: 10.1507/endocrj.50.613.
Objective of the present study is to determine the estrogenic effect of isoflavone on vaginal epithelia and bone metabolism in early postmenopausal women. Twenty-two postmenopausal women were randomly assigned to either a group that was given isoflavone extract (61.8 mg) for three months or a control group that was given placebo. We measured the L2-4 bone mineral density (BMD) before and 3 months after treatment by dual X-ray absorptiometry (DXA). Blood and urine samples were obtained from the women before and 3 months after treatment. We measured FSH using radioimmunoassay and, urinary pyridinoline and deoxypyridinoline levels by HPLC. For endocrine cytology, vaginal smears were collected before and 3 months after the treatment. Three months after the treatment, the serum FSH levels and the BMD values did not significantly differ between the two groups. Urinary excretion of isoflavone was significantly higher in the group given isoflavone compared with that given placebo (p<0.03). Numbers of parabasal and intermediate types of cells were significantly decreased (58.2 +/- 12.4% to 25.0 +/- 10.7%; p<0.05) and increased (24.1 +/- 8.7% to 63.7 +/- 10.7%; p<0.05), respectively in the isoflavone group, but remained unchanged in the control group. Urinary pyridinoline excretion was significantly decreased (49.6% vs. before, p<0.01 by paired t-test) in the isoflavone group. The intake of 60 mg of isoflavone daily for 3 months produced maturational changes of vaginal epithelia without affecting serum FSH levels, and could possibly slow down bone turnover rates as judged by decreased urinary pyridinoline excretion.
本研究的目的是确定异黄酮对绝经后早期女性阴道上皮和骨代谢的雌激素样作用。22名绝经后女性被随机分为两组，一组服用异黄酮提取物（61.8毫克），为期三个月，另一组为对照组，服用安慰剂。我们通过双能X线吸收法（DXA）测量了治疗前及治疗3个月后的L2 - 4骨密度（BMD）。在治疗前及治疗3个月后采集了这些女性的血液和尿液样本。我们使用放射免疫分析法测量促卵泡激素（FSH），并通过高效液相色谱法测量尿吡啶啉和脱氧吡啶啉水平。对于内分泌细胞学检查，在治疗前及治疗3个月后采集阴道涂片。治疗3个月后，两组之间的血清FSH水平和BMD值无显著差异。与服用安慰剂组相比，服用异黄酮组的异黄酮尿排泄量显著更高（p<0.03）。异黄酮组中基底细胞和中层细胞数量分别显著减少（从58.2±12.4%降至25.0±10.7%；p<0.05）和增加（从24.1±8.7%增至63.7±10.7%；p<0.05），而对照组则保持不变。异黄酮组的尿吡啶啉排泄量显著降低（配对t检验，与治疗前相比降低49.6%，p<0.01）。每天摄入60毫克异黄酮，持续3个月，可使阴道上皮发生成熟变化，而不影响血清FSH水平，并且从尿吡啶啉排泄量减少判断，可能会减缓骨转换率。
