Kent S, Kelley K W, Dantzer R
INRA-INSERM U176, Bordeaux, France.
Neurosci Lett. 1992 Sep 28;145(1):83-6. doi: 10.1016/0304-3940(92)90209-p.
To investigate the role of interleukin-1 (IL-1) in the decrease in food-motivated behavior after peripheral administration of lipopolysaccharide (LPS), rats trained to press a lever for food on a fixed ratio 10 schedule were pre-treated with a recombinant human IL-1 receptor antagonist (IL-1ra). This endogenous cytokine has been shown to block most of the inflammatory and immune effects of IL-1 both in vitro and in vivo. Intraperitoneal (i.p.) injection of LPS (400 micrograms/kg) decreased operant responding for food to 30-60% of baseline for 1-4 h. Response rates gradually recovered, but were still below control levels 8 and 24 h post-injection. Neither i.p. (8 mg/kg) nor intracerebroventricular (288 micrograms/kg) administration of IL-1ra blocked the effects of peripherally administered LPS on food-motivated behavior. These results suggest that the effects of LPS on this behavior are not mediated by the release of IL-1.
为研究白细胞介素-1（IL-1）在腹腔注射脂多糖（LPS）后食物驱动行为减少中的作用，对按固定比率10训练以按压杠杆获取食物的大鼠，预先给予重组人IL-1受体拮抗剂（IL-1ra）。这种内源性细胞因子已被证明在体外和体内均可阻断IL-1的大多数炎症和免疫效应。腹腔注射LPS（400微克/千克）可使食物操作性反应在1至4小时内降至基线水平的30%-60%。反应率逐渐恢复，但在注射后8小时和24小时仍低于对照水平。腹腔注射（8毫克/千克）或脑室内注射（288微克/千克）IL-1ra均不能阻断外周注射LPS对食物驱动行为的影响。这些结果表明，LPS对该行为的影响并非由IL-1的释放介导。
