Hammer K A, Dry L, Johnson M, Michalak E M, Carson C F, Riley T V
Discipline of Microbiology, School of Biomedical and Chemical Sciences, The University of Western Australia, Crawley, Western Australia, Australia.
Oral Microbiol Immunol. 2003 Dec;18(6):389-92. doi: 10.1046/j.0902-0055.2003.00105.x.
The in vitro activity of Melaleuca alternifolia (tea tree) oil against 161 isolates of oral bacteria from 15 genera was determined. Minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) ranged from 0.003 to 2.0% (v/v). MIC90 values were 1.0% (v/v) for Actinomyces spp., Lactobacillus spp., Streptococcus mitis and Streptococcus sanguis, and 0.1% (v/v) for Prevotella spp. Isolates of Porphyromonas, Prevotella and Veillonella had the lowest MICs and MBCs, and isolates of Streptococcus, Fusobacterium and Lactobacillus had the highest. Time kill studies with Streptococcus mutans and Lactobacillus rhamnosus showed that treatment with > or = 0.5% tea tree oil caused decreases in viability of >3 log colony forming units/ml after only 30 s, and viable organisms were not detected after 5 min. These studies indicate that a range of oral bacteria are susceptible to tea tree oil, suggesting that tea tree oil may be of use in oral healthcare products and in the maintenance of oral hygiene.
测定了互叶白千层（茶树）油对来自15个属的161株口腔细菌的体外活性。最低抑菌浓度（MIC）和最低杀菌浓度（MBC）范围为0.003%至2.0%（v/v）。放线菌属、乳酸杆菌属、缓症链球菌和血链球菌的MIC90值为1.0%（v/v），普雷沃菌属的MIC90值为0.1%（v/v）。卟啉单胞菌属、普雷沃菌属和韦荣球菌属的分离株MIC和MBC最低，链球菌属、梭杆菌属和乳酸杆菌属的分离株最高。对变形链球菌和鼠李糖乳杆菌的时间杀菌研究表明，用≥0.5%茶树油处理仅30秒后，菌落形成单位/毫升的活力就下降了>3个对数，5分钟后未检测到活菌。这些研究表明，一系列口腔细菌对茶树油敏感，提示茶树油可能用于口腔保健产品和维持口腔卫生。
