Banes-Marshall L, Cawley P, Phillips C A
Department of Microbiology, Kettering General Hospital NHS Trust, Northamptonshire, UK.
Br J Biomed Sci. 2001;58(3):139-45.
This study investigates the in vitro activity of tea tree oil (TTO) against a range of wild strains of microorganisms isolated from clinical specimens of leg ulcers and pressure sores. The antimicrobial effectiveness of TTO is determined in terms of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) or minimum fungicidal concentration (MFC). The isolates include methicillin-resistant Staphylococcus aureus (MRSA), S. aureus, faecal streptococci, beta-haemolytic streptococci, coagulase-negative staphylococci, Pseudomonas spp. and coliform bacilli. Eleven Candida spp. isolates from skin and vaginal swabs also are tested. Using an agar dilution assay, the MICs of TTO in 88 out of 90 isolates was 0.5-1.0% (v/v), whilst with P. aeruginosa it was >2% (v/v). A broth microdilution method was used to determine MIC and minimum cidal concentration (MCC) of 80 isolates. In 64 isolates, TTO produced an inhibitory and cidal effect at 3% and 4% (v/v), respectively. S. aureus and Candida spp. were the most susceptible to TTO, with MICs and MBCs of 0.5% and 1%, respectively. P. aeruginosa and the faecal streptococci isolates, with MICs and MBCs of >8%, were resistant to TTO.
本研究调查了茶树油（TTO）对从腿部溃疡和压疮临床标本中分离出的一系列野生微生物菌株的体外活性。根据最低抑菌浓度（MIC）和最低杀菌浓度（MBC）或最低杀真菌浓度（MFC）来确定TTO的抗菌效果。分离菌株包括耐甲氧西林金黄色葡萄球菌（MRSA）、金黄色葡萄球菌、粪链球菌、β-溶血性链球菌、凝固酶阴性葡萄球菌、假单胞菌属和大肠杆菌。还对从皮肤和阴道拭子中分离出的11种念珠菌属菌株进行了测试。采用琼脂稀释法，90株分离菌株中有88株TTO的MIC为0.5 - 1.0%（v/v），而铜绿假单胞菌的MIC > 2%（v/v）。采用肉汤微量稀释法测定80株分离菌株的MIC和最低杀菌浓度（MCC）。在64株分离菌株中，TTO分别在3%和4%（v/v）时产生抑制和杀菌作用。金黄色葡萄球菌和念珠菌属对TTO最敏感，MIC和MBC分别为0.5%和1%。铜绿假单胞菌和粪链球菌分离株的MIC和MBC > 8%，对TTO耐药。
