Rubinstein P, Falk C, Martin M, Nicholson J, Suciu-Foca N
Transplant Proc. 1977 Dec;9(4):1747-9.
LCA, a rapid and efficient computer method to allocate blastogenic responses to positive and negative clusters, is described. The method involves the selection of a partition level for an ordered array of values, such that the resulting two clusters have minimal "within-cluster" variances. Individuals to whom the program assigned the same two HLA-D antigens were MLC-identical (cluster 0) in 50% of the cases. This proportion was lower for HLA-D-identical pairs that typed for Dw4 and/or Dw7, indicating that these antigens are less well defined than the rest. PLTs were less efficient than HTCs in HLA-D typing due to the induction of extra (non-HLA-D determined) responses in the higher cluster.
本文描述了一种名为LCA的快速高效的计算机方法，用于将增殖反应分配到阳性和阴性簇中。该方法涉及为有序值数组选择一个划分级别，以使所得的两个簇具有最小的“簇内”方差。在50%的病例中，程序分配了相同两种HLA-D抗原的个体在混合淋巴细胞培养（MLC）中是相同的（簇0）。对于检测到Dw4和/或Dw7的HLA-D相同对，这一比例较低，表明这些抗原的定义不如其他抗原明确。由于在较高簇中诱导了额外的（非HLA-D决定的）反应，血小板在HLA-D分型中比混合淋巴细胞培养物效率更低。
