Shumakov V I, Onishchenko N A, Rasulov M F, Krasheninnikov M E, Zaidenov V A
Institute of Transplantology and Artificial Organs, Ministry of Health of Russian Federation, Moscow.
Bull Exp Biol Med. 2003 Aug;136(2):192-5. doi: 10.1023/a:1026387411627.
Regeneration of deep burn wounds after transplantation of allogenic and autogenic fibroblast-like bone marrow mesenchymal stem cells and embryonic fibroblasts on burn surface was studied in 40 Wistar rats. Transplantation of allogenic and autogenic fibroblast-like bone marrow mesenchymal stem cells and transplantation of embryonic fibroblasts decreased cell infiltration of the wound and accelerated the formation of new vessels and granulation tissue in the wound in comparison with the control (burn wounds without cell transplantation). Regeneration processes were most active after transplantation of fibroblast-like bone marrow mesenchymal stem cells, in particular, autogenic cells, which was confirmed by more rapid decrease in burn surface area. Wound healing after transplantation of fibroblast-like bone marrow mesenchymal cells and embryonic fibroblasts was associated with long functioning of transplanted cells (as was shown by staining for beta-galactosidase, the cells were transfected with an adenovirus vector carrying the marker gene). It is hypothesized that more rapid regeneration of burn wounds after transplantation of fibroblast-like bone marrow mesenchymal stem cells was due to low differentiation of these cells in comparison with embryonic fibroblasts.
在40只Wistar大鼠中研究了将同种异体和自体成纤维细胞样骨髓间充质干细胞以及胚胎成纤维细胞移植到烧伤表面后深度烧伤创面的再生情况。与对照组（未进行细胞移植的烧伤创面）相比，同种异体和自体成纤维细胞样骨髓间充质干细胞的移植以及胚胎成纤维细胞的移植减少了创面的细胞浸润，并加速了创面新血管和肉芽组织的形成。成纤维细胞样骨髓间充质干细胞移植后，尤其是自体细胞移植后，再生过程最为活跃，这一点通过烧伤表面积更快减小得到证实。成纤维细胞样骨髓间充质细胞和胚胎成纤维细胞移植后的伤口愈合与移植细胞的长期功能有关（通过β-半乳糖苷酶染色显示，细胞用携带标记基因的腺病毒载体进行了转染）。据推测，成纤维细胞样骨髓间充质干细胞移植后烧伤创面再生更快是由于这些细胞与胚胎成纤维细胞相比分化程度较低。
