Schlücker Sebastian, Schaeberle Michael D, Huffman Scott W, Levin Ira W
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0510, USA.
Anal Chem. 2003 Aug 15;75(16):4312-8. doi: 10.1021/ac034169h.
Three different Raman microspectroscopic imaging methodologies using a single experimental configuration are compared; namely, point and line mapping, as representatives of serial imaging approaches, and direct or wide-field Raman imaging employing liquid-crystalline tunable filters are surveyed. Raman imaging data acquired with equivalent low-power 514.5-nm laser excitation and a cooled CCD camera are analyzed with respect to acquisition times, image quality, spatial resolution, intensity profiles along spatial coordinates, and spectral signal-to-noise ratios (SNRs). Point and line mapping techniques provide similar SNRs and reconstructed Raman images at spatial resolutions of approximately 1.1 microm. In contrast, higher spatial resolution is obtained by direct, global imaging (approximately 313 nm), allowing subtle morphological features on test samples to be resolved.
比较了使用单一实验配置的三种不同拉曼显微光谱成像方法；即作为串行成像方法代表的点和线映射，以及采用液晶可调滤光片的直接或宽场拉曼成像。分析了使用等效低功率514.5 nm激光激发和冷却电荷耦合器件相机获取的拉曼成像数据的采集时间、图像质量、空间分辨率、沿空间坐标的强度分布以及光谱信噪比（SNR）。点和线映射技术在空间分辨率约为1.1微米时提供相似的SNR和重建拉曼图像。相比之下，直接全局成像可获得更高的空间分辨率（约313 nm），从而能够分辨测试样品上的细微形态特征。
