Shin Kyoung-Jin, Kwon Byung-Ki, Lee Sang-Seob, Yoo Ji-Eun, Park Myung Jin, Chung Ukhee, Lee Hwan Young, Han Gil-Ro, Choi Jong-Hoon, Kim Chong-Youl
Department of Forensic Medicine, College of Medicine, Yonsei University, 134 Shinchon-Dong, Seodaemun-Gu, 120-752 Seoul, Korea.
Int J Legal Med. 2004 Feb;118(1):37-40. doi: 10.1007/s00414-003-0415-2. Epub 2003 Nov 22.
The five X-chromosomal short tandem repeats (STRs) GATA172D05, HPRTB, DXS8377, DXS101 and HumARA were analyzed in 150 males and 150 females from Korea. Markers were amplified in a quadruplex and a monoplex PCR reaction with fluorescently labeled primers. For accurate and reproducible STR typing, sequenced allelic ladders were constructed and a Genotyper macro was programmed. Some differences were found on comparing the allele frequencies of Koreans with those of other populations in DXS8377, DXS101 and HumARA. The forensic efficiency parameters showed that the five X-linked STRs are highly informative for forensic application in Koreans.
对来自韩国的150名男性和150名女性分析了五个X染色体短串联重复序列(STR),即GATA172D05、HPRTB、DXS8377、DXS101和HumARA。使用荧光标记引物,通过四重PCR反应和单重PCR反应对标记物进行扩增。为了实现准确且可重复的STR分型,构建了测序等位基因阶梯,并编写了基因分型宏程序。在比较韩国人与其他群体在DXS8377、DXS101和HumARA中的等位基因频率时发现了一些差异。法医效率参数表明,这五个X连锁STR在韩国人的法医应用中具有高度信息性。