Tolstikov Vladimir V, Lommen Arjen, Nakanishi Kazuki, Tanaka Nobuo, Fiehn Oliver
Department Lothar Willmitzer, Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany.
Anal Chem. 2003 Dec 1;75(23):6737-40. doi: 10.1021/ac034716z.
Application of C18 monolithic silica capillary columns in HPLC coupled to ion trap mass spectrometry detection was studied for probing the metabolome of the model plant Arabidopsis thaliana. It could be shown that the use of a long capillary column is an easy and effective approach to reduce ionization suppression by enhanced chromatographic resolution. Several hundred peaks could be detected using a 90-cm capillary column for LC separation and a noise reduction and automatic peak alignment software, which outperformed manual inspection or commercially available mass spectral deconvolution software.
研究了C18整体硅胶毛细管柱在与离子阱质谱检测联用的高效液相色谱中的应用，以探究模式植物拟南芥的代谢组。结果表明，使用长毛细管柱是通过提高色谱分辨率来减少电离抑制的一种简便有效的方法。使用一根90厘米的毛细管柱进行液相色谱分离，并结合降噪和自动峰对齐软件，可以检测到数百个峰，其性能优于人工检查或市售的质谱去卷积软件。
